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Chromosome Aberration in Suspension Culture of Ginseng(Panax ginseng C. A. Meyer) Callus

인삼 캘러스 현탁배양에 있어서의 염색체 이상

  • Park, Jong-Bum (Department of Biological Sciences, Silla University)
  • 박종범 (신라대학교 생물과학과)
  • Published : 2006.12.31

Abstract

This study was to examine the variations of chromosome number and the ranges of variety in the suspension culture of ginseng (Panax ginseng C. A. Meyer) callus cell, and the effect of plant hormones for the chromosome aberration. Plant hormones added with MS medium in the suspension culture were 2,4-D, kinetin, and 2,4-D+kinetin and concentration of the plant hormones were $1000{\mu}M$ and $0.1\;{\mu}M$ respectively. As a result of these experiment the following conclusion has been obtained. Media contained with 2,4-D+kinetin in $10{\mu}M$ concentration was very effective in the suspension culture result from 26.4% mitosis frequency, and found the various variation of chromosome number. Variety of chromosome number was diversed ($9\sim110$), espicially frequency of hypohaploid and hyperhaploid cells were very higher than hyperdiploid cells. In this experiments, it is suggested that $10{\mu}M$ 2,4-D+kinetin added with medium in the suspension culture of ginseng callus was effect in the variations of chromosome number.

Keywords

Chromosome aberration;Suspension culture;Ginseng;Callus

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