Improvement of a Screening System for Environmental Mutagens by Means of a Specific Locus Mutation of the Silkworm, Bombyx mori

누에를 이용한 환경 변이원 검색계 재선

  • Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Jong-Gill (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Choi, Ji-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
  • 윤형주 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김삼은 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김종길 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 최지영 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과)
  • Published : 2006.12.30

Abstract

The efficiency to detect mutagenicity of the system using a specific locus mutation of Bombyx mori was examined and improved. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among tested four mutagens, MMC had specially high sensitivity in the oocytes of silkworm and EMS had in the spermatozoa. PCB and dioxin showed a positive effect in both the oocytes and spermatozoa. In a consequence of sensitivity of mutagen by mating number of male moth of B. mori, treated mutagen, there was no difference between one mating - and three mating - male moth in sensitivity of mutagen. Sun3ho, B. mori variety, which showed high sensitivity to mutagens was improved in the major characteristics by crossing of C5 and N12.

Keywords

Mutagenicity;Bombyx mori;Specific locus mutation;Egg colour

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