The Molecular Characterization of Serogroup C Neisseria meningitidis Strains Circulating in Beijing

  • Zhang, Tie-Gang (Institute of Immunization and Prevention, Beijing Center for Disease Prevention and Control) ;
  • He, Jing-Guo (Institute of Immunization and Prevention, Beijing Center for Disease Prevention and Control) ;
  • He, Xiong (Institute of Immunization and Prevention, Beijing Center for Disease Prevention and Control) ;
  • Chen, Li-Juan (Institute of Immunization and Prevention, Beijing Center for Disease Prevention and Control) ;
  • Shao, Zhu-Jun (National Institute for Communicable Disease Control and Prevention) ;
  • Sun, Mei-Ping (Institute of Immunization and Prevention, Beijing Center for Disease Prevention and Control)
  • Published : 2006.12.31

Abstract

The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.12-1,16-8. All of the strains expressed class 3 PorB protein. Among the five pulsed-field gel electrophoresis patterns observed, pattern III predominated.

References

  1. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing. Supplement M100-S15. Clinical and Laboratory Standards Institute, Wayne, Pa, USA
  2. Goulding, J.N., J.V. Hookey, J. Stanley, W. Olver, K.R. Neal, D.A. Ala'Aldeen, and C. Arnold. 2000. Fluorescent amplified- fragment length polymorphism genotyping of Neisseria meningitidis identifies clones associated with invasive disease. J. Clin. Microbiol. 38, 4580-4585
  3. Hu, X.J., X.W. Li, Y.D. Ji, W. Xi, and L.H. Gao 1991. Study on periodically prevalent features for epidemic cerebrospinal meningitis in China. Chinses J. Epidemiol. 12, 136-139
  4. Hu, X.J. 2001. Epidemiological surveillance and prophylaxis of epidemic cerebrospinal Meningitis. Chinese J. Vaccin. Immun. 7, 300-303
  5. Maiden, M.C., J.A. Bygraves, E. Feil, G. Morelli, J.E. Russell, R. Urwin, Q. Zhang, J. Zhou, K. Zurth, D.A. Caugant, I.M. Feavers, M. Achtman, and B.G. Spratt. 1998. Multilocussequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. 95, 3140-3145
  6. Morelli, G., B. Malorny, K. Muller, A. Seiler, J.F. Wang, J. del Valle, and M. Achtman. 1997. Clonal descent and microevolution of Neisseria meningitidis during 30 years of epidemic spread. Mol. Microbiol. 25, 1047-1064 https://doi.org/10.1046/j.1365-2958.1997.5211882.x
  7. Popovic, T., G.W. Ajello, and R. Facklam. 1999. Manual for the laboratory diagnosis of meningitis caused by Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae. World Health Organization, Geneva, Switzerland
  8. Sacchi, C.T., A.P. Lemos, A.M. Whitney, C.A. Solari, M.E. Brandt, C.E. Melles, C.E. Frasch, and L.W. Mayer. 1998a. Correlation between serological and sequencing analyses of the PorB outer membrane protein in the N. meningitidis serotyping system. Clin. Diagn. Lab. Immun. 5, 348-354
  9. Sacchi, C.T., A.P. Lemos, M.E. Brandt, A.M. Whitney, C.E. Melles, C.A. Solari, C.E. Frasch, and L.W. Mayer. 1998b. Proposed standardization of Neisseria meningitidis PorA variable-region typing nomenclature. Clin. Diagn. Lab. Immun. 5, 845-855
  10. Shao, Z.J., W. Li, J. Ren, X.F. Liang, L. Xu, B.W. Diao, M.C. Li, M.J. Lu, H.Y. Ren, Z.G. Cui, B.Q. Zhu, Z.W. Dai, L. Zhang, X. Chen, B. Kan, and J.G. Xu. 2006. Identification of a new Neisseria meningitidis serogroup C clone from Anhui province, China. Lancet 367, 419-423 https://doi.org/10.1016/S0140-6736(06)68141-5
  11. Zhang, T.G., X. He, L.J. Chen, J.G. He, M. Luo, J. Yang, Z.J. Shao, and M.P. Sun. 2006. Laboratory confirmation of a suspicious meningococcal meningitis death case. J. Microbiol. 44, 457-460