Antioxidant Effect of Vitamin E on Vascular Endothelial Cells Damaged by Reactive Oxygen Species

활성산소종으로 손상된 혈관내피세포에 대한 Vitamin E의 항산화 효과

  • Suk, Seung-Han (Department of Neurology, Wonkwang University Sanbon Medical Center)
  • 석승한 (원광대학교 의과대학 산본병원 신경과학교실)
  • Published : 2006.06.25


In order to examine the injury of vascular endothelial cells related with oxidative stress of reactive oxygen species(ROS), mophological changes of vascular endothelial cells were observed by light microscope after bovine pulmonary vascular endothelial cell line (BPVEC) was treated with 15 uM of hydrogen peroxide. In addition, the effect of vitamin E against ROS-induced oxidative stress was examined by light microscope. In this study, the cell number of BPVEC treated with ROS has significantly decreased than that of control, and the loss of cytoplasmic processes and cell swelling were observed in BPVEC treated with ROS. Whereas, cell number of BPVEC treated with vitamin E has significantly increased than that of BPVEC treated with ROS and also, cytoplasmic processes of BPVEC treated with vitamin E were preserved as control. These findings suggested that not only did ROS induce damage of BPVES by decrease of cell number, loss of cytoplasmic processes and cell swelling, but vitamin E also has protective effect against ROS-induced oxidative stress in cultures of BPVEC.


  1. Libby, P., Deanfiled, J.E. A CME monograph : Atherosclerosis and vascular disease Academy for Health Education, 1-28, 2002
  2. Wautier, J.L., Zoukourian, C., Chappey, O. Receptor-mediated endothelial cell dysfunction in diabetic vasculopathy: soluble receptor for advanced glycation end products blocks hyperpermeability in diabetic rats. J Clin Invest 97:238-243, 1996
  3. Sellke, F.M., Amstrong, M.L., Harrison, D.G. Endothelium- dependent vascular relaxation is abnormal in the coronary microcirculation of atheosclerotic primates. Circ Res 81:1586-1593, 1990
  4. Shimokawa, H., Vanhoutte, P.M. Dietary cod-liver oil improves endothelium-dependent responses in hypercholesterolemic and atherosclerotic porcine coronary arteries. Circulation 78:1421-1430, 1988
  5. Beckman, J.S., Koppenol, W.H. Nitric oxide, superoxide and peroxynitrite; the good, the bad and ugly. Am J Physiol 271:1424-1437, 1996
  6. Toda, N., Okamura, T. Role of nitrix oxide in neurally induced cerebroarterial relaxation. J Pharmacol Exp Ther 258:1027-1032, 1991
  7. Kuo, L., Davis, M.J., Cannon, M.S. Pathophysiological consequences of atherosclerosis extend into the coronary microcirculation. Restoration of endothelium-dependent responses by L-arginine. Cir Res 70:465-476, 1992
  8. Mangan, D.F., Welch, G.R., Wahl, S.M. Lipopolysaccaride, tumor necrosis factor-alpha, and IL-1 beta prevent programmed cell death (apoptosis) in human peripheral blood monocytes. J Immunol. 146:1541-1546, 1993
  9. Sawada, M., Kondo, N., Suzumura, A., Marunouchi, T. Production of tumor necrosis factor-alpha by microglia and astrocytes in culture. Brain Res 491:394-397, 1989
  10. Ganz, M.B., Saksa, B., saxena, R., Hawkins, K., Sedor, J.R. PDGF and IL-1 induce and activate specific protein kinase C isoforms in mesangial cells. Am J Physiol 217:F108-F113, 1996
  11. Bevilacqua, M.P. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol 11:767-804, 1993
  12. Takahara, N., Kashiwagi, A., Nishio, Y., Harada, N., Kojima, H., Maegawa, H., Kikawa, R. Oxidized lipoproteins found in patients with NIDDM stimulateradical-induced monocyte chemoattractant proten-1 mRNA expression in cultured human endothelial cells. Diabetologia 662-670, 1997
  13. Harrison, D.G. Endothelial dysfunction in atherosclerosis. Basic Res Cardiol 89(suppl):87-102, 1994
  14. Kapor-Drezgic, J., Zhou, X., Whiteside, C. Messandial cell PKC isoform accumulation and membrane association in high glucose(Abstract). J Am Soc Nephrol 8:640A, 1997
  15. Jacobson, J.M., Michael, J.R., Jafri, M.H., Gurtner, G.H. Antioxidants and antioxidant enzymes protect against pulmonary oxygen toxicity in the rabbit. J Appl Physiol 68:1252-1259, 1990
  16. Chuyen, N.V., Utsunomiya, N., Hodaka, A., Kato, H. Antioxidative effect of Maillard reaction products in vivo. in the "The Maillard reaction in food processing, human nutrition and physiology". ed. PA Pinot Birkhauser Verlag Basel, pp 285-290 1990
  17. Rosen, D., Siddique, T., Patterson, D., Figlewiez, D., Sapp, P., Hentati, A., Donaldson, D., Goto, J., O Regan, J., Deng, H., Rahmani, Z., Krrizus, A. et al : Mutation in Cu /Zn superoxide dismutase gene are associated wigh familial amyotrophic lateral sclerosis. Nature (London) 362:59-62, 1993
  18. Kim, H.S., Lee, Y.S., Oh, S.K., Lee, K.C., Lee, G.M., Lee, J., Lee, S.B., Kim, J.H., Yu, J.K., Kang, Y.S., Kim, S.S., Song, H.J., Park, S.T. Effect of Ramulus et Uncus Uncariae on Glucose Oxidase-Induced Toxicity in Cultured Cerebral Neurons. Korean J Oriental Physiol & Pathol 16(5):1016-1019, 2002
  19. Hayase, F., Hirashima, S., Okamoto, G., Kato, H. Scavenging of active oxygens by melanoidin. Agric Biol Chem 53:3383-3389, 1989
  20. Nistico, G., Ciriolo, M.R., Fiskin, D., Lannone, M., DeMartino, A., Rotilio, G. NGF restores decrease in catalase activity and increases superoxide dismutase and glutathione peroxidase activity in the brain of age rat. Free Rad Biol Med 12:177-182, 1992.
  21. Reiter, R.J. Oxidative processes and antioxidative defense mechanism in the aging brain. FASEB J 9:526-533, 1995
  22. Pedder, S.C., Wilcox, R.I., Johnson, D.D. Attenuation of febrile seizures in epileptic chicks by N-methy-D-aspartate receptor antagonist. Can J Physiol Pharmacol 68:84-88, 1990
  23. Goldstein, R.H., Poliks, C.F., Pilch, p.F., Smith, B.D. Fine A. Stimulation of collagen formation by insulin and insulin-like growth factor I in cultures of human lung fibroblasts. Endocrinology 124(2):964-970, 1989
  24. Yamamoto, M., Scima, T., Uozumi, T., Yamada, K., Kawasaki, T. A possible role of lipid peroxidation in cellular damages caused by cerebral ischemia and protective effect of alpatocopherol administration. Stroke 14:977-982, 1983
  25. Cho, C.G., Kim, H.M., Park, S.T. Effect of Vitamin E on Cultured Mouse Cerebral Neurons Damaged by Oxidative stress. Kor J Gerontol 8(3):17-22, 1988
  26. Loft, S., Astrup, A., Buemann, B., Poulsen, H.E. Oxidative DNA damage correlates with oxygen consumption in humans. FASEB J 8:534-537, 1994
  27. Mosmann, T. Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxic assays. J Immunol Methods 65:55-63, 1983
  28. Jain, S.K. Hyperglycemic can cause membrane lipidperoxidation and osmic fragility in human red blood cells. J Biolo Chem 264:21340-21345, 1989
  29. Kaneko, M., Elmban, V., Dhalla, N.S. Mechanism for depression of heart sarcolemmal $Ca^{2+}$ pump by oxygen free radicals. Am J Physiol 261:4948-4955, 1989
  30. Toraason, M., Breitenstein, M. Intercellular calcium transients in cardiac myocytes exposed to carbon tetrachloride (Abstract). Toxicologyist 11:310, 1991