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Molecular Characterization of Hanwoo Glucose Transporter 4 Gene

한우 Glucose Transporter 4 유전자의 분자생물학적 해석

  • Lee, S.M. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Jeong, Y.H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Kim, H.M. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Park, H.Y. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Moon, S.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Chung, E.R. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University) ;
  • Kang, M.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
  • 이상미 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 정영희 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 김혜민 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 박효영 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 윤두학 (농촌진흥청 축산연구소) ;
  • 문승주 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 정의룡 (상지대학교 생명자원과학대학 생명공학과) ;
  • 강만종 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부)
  • Published : 2005.12.31

Abstract

The uptake of glucose for metabolism and growth is essential to most animal cells and is mediated by glucose transport protein. In the glucose transport protein family, GLUT4 plays a key role in cellular glucose uptake stimulated by insulin in skeletal muscles and adipose tissue in rodents and human. In this studies, we reported the identification, characterization, and expression of Hanwoo GLUT4 gene. The Hanwoo GLUT4 cDNA includes a 1527 bp open reading frame encoding a protein of 509 amino acids. The GLUT4 amino acid sequences of the Hanwoo show strong conservation with the corresponding sequences reported in other species. The highest mRNA expression of GLUT4 was detected in heart and lower expression was detected in rib meat, sirloin, and colon. We confirmed the expression of GLUT4 in the subcutaneous and small intestinal adipose tissue using RT-PCR. To investigate the expression of GLUT4 in the bovine intramuscular adipose differentiation, fibroblast-like cells were isolated from the sirloin of Hanwoo bull aged 12 months by collagenase digestion of minced tissue and cultured with activators of PPAR gamma. We identified that GLUT4 mRNA expression decreased during differentiation of preadipocytes into adipocyte in Korean cattle. These results indicated that function of GLUT4 in bovine adipose tissue was different from that of mouse and human.

Keywords

Adipocyte differentiation;Bovine;Intramuscular preadipocyte

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