Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass

켄터키 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향

  • Lee, K.W. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, S.H. (Division of Applied Life Science. Gyeongsang National University) ;
  • Lee, D.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo, H.S. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, D.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, M.S. (Division of Forest Science, Gyeongsang National University) ;
  • Won, S.H. (Institute of Agri. Sci. & Tech., Kyungpook National University) ;
  • Seo, S. (National Livestock Research Institute, R.D.A.) ;
  • Lee, B.H. (Division of Applied Life Science, Gyeongsang National University)
  • 이기원 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 김도현 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 원성혜 (경북대학교 농업과학기술연구소) ;
  • 서성 (농촌진흥청 축산연구소) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Published : 2005.12.31


In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.


Embryogenic callus;Forage crop;Plant tissue culture;Transformation


  1. Altpeter, F. and Xu, J. 2000. Rapid production of transgenic turfgrass(Festuca rubra L.) plants. Plant Physiol 157:441-448
  2. Bai, Y. and Qu. R. 2000. An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue(Festuca arundinacea Schreb.) cultivars. Grass Forage Sci 55:326-330
  3. Chu, C. C., Wang, C. S., Sun, C. C, Hsu, C., Yin, K. C., Chu, C. Y. and Bi, F. Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668
  4. Forster, J. W. and Spangenberg, G. 1999. Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow, J. K.(Ed.), Genetic engineering: principles and methods, Vol. 21, Kluwer Academic Publishers, New York, p. 191
  5. Ke, S. and Lee, C. W. 1996. Plant regeneration in Kentucky bluegrass(Poa pratensis L.) via coleoptile tissue cultures. Plant Cell Rep. 15:882-887
  6. Krans, J. V. 1981. Cell culture of turfgrass. pp. 27-33
  7. Lee, S. H., Lee, D. G., Kim, J. S. and Lee, B. H. 2003. High-frequency plant regeneration from mature seed-derived callus culture of orchardgrass. Kor J Plant Biotechnology 30:341-346
  8. McKersie, B. D. 1997. Improving forage production systems using biotechnolgy. In: McKersie, B. D. and Brown, D. C W.(Eds), Biotechnology in Agriculture Series, No. 17, CAB International, Wallingford, p. 3
  9. Murashige, T. and Skoog, F. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
  10. Nielsen, K. A. and Knudsen, E. 1993. Regeneration of green plants from embryogenic suspension culture of Kentucky bluegrass(Poa pratensis L.). Plant Cell Rep 12:537-540
  11. Rim, Y. W., Kim, K. Y., Choi, K. J., Sung, B. R. and Shin, J. S. 2000. Callus induction from seeds of Italian ryegrass and plant regeneration. J. Kor Grassland Sci 20:25-0
  12. SAS. 1999. SAS/STAT Software for PC. Release 8.1, SAS institute, Cary, NC, U.S.A
  13. Schenk, R. U. and Hildebrandt, A. C. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-04
  14. Spangenberg, G., Wang, Z. Y. and Potrykus, I. 1998. Biotechnology in forage and turf grass improvement. In: Frankel et al(Eds), Monographs on theoretical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, p. 192
  15. Van Ark, H. F., Zaal, M. A. C. M., Creemers-Molenaar, J. and Van der Valk, P. 1991. Improvement of tissue culture response of seed-derived callus cultures of Kentucky bluegrass. Plant Vell Tiss Org Cult 27:275-280
  16. Van Wijk, A. J. P., Boonman, J. G. and Rumball, W. 1993. Achievements and prospectives in the breeding of forage grasses and legumes. In: Baker, M. J.(Eds), Grasslands for our world, SIR, Wellington, p. 116
  17. Vasil, V. and Vasil, I. K. 1984. Induction and maintenance of embryogenic callus cultures of Gramineae. In: Vasil IK(eds), Cell culture and somatic cell genetics of plants, Vol 1. Academic Press, Orlando, pp. 36-42
  18. Wang, Z. Y., Nagel, J., Potrykus, I. and Spangenberg, G. 1993. Plants from cell suspension-derived protoplasts in Lolium species. Plant Sci 94: 179-193
  19. Ye, X., Wang, Z. Y., Wu, X., Potrykus, I. and Spangenberg, G. 1997. Transgenic Italian ryegrass (Latium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Rep 16:379-384
  20. Van der Valk, P., Ruis, F., Tettelaar-Schrier. A. M. and Van der Velde, C. M. 1995. Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass, the effect of benzyladenine. Plant Cell Tiss Org Cult 40:101-103