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Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass

켄터키 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향

  • Lee, K.W. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, S.H. (Division of Applied Life Science. Gyeongsang National University) ;
  • Lee, D.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo, H.S. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, D.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, M.S. (Division of Forest Science, Gyeongsang National University) ;
  • Won, S.H. (Institute of Agri. Sci. & Tech., Kyungpook National University) ;
  • Seo, S. (National Livestock Research Institute, R.D.A.) ;
  • Lee, B.H. (Division of Applied Life Science, Gyeongsang National University)
  • 이기원 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 김도현 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 원성혜 (경북대학교 농업과학기술연구소) ;
  • 서성 (농촌진흥청 축산연구소) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Published : 2005.12.31

Abstract

In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.

Keywords

Embryogenic callus;Forage crop;Plant tissue culture;Transformation

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