Effects of Dietary Eucommia ulmoides Leaves on Nutrient Digestibility and Ruminal Fermentation Characteristics in Sheep

두충잎의 급여가 면양의 영양소 이용율과 반추위내 발효특성에 미치는 영향

  • Kim, J.H. (Korea Applied Microorganism Industrial Research) ;
  • Ko, Y.D. (College of Agriculture and Life Science, Gyeongsang National University)
  • 김재황 (한국응용미생물산업연구소) ;
  • 고영두 (경상대학교 농업생명과학대학)
  • Published : 2005.12.31


This study was conducted to examine the nutrient digestibility and ruminal fermentation characteristics in sheep fed diets containing four substitution levels of E. ulmoides leaves for rice straw. For the experiment, they were given a basal diet consisting of rice straw and concentrate at a 3:7 ratio (DM basis). The treatments were designed as a 4$\time$4 Latin square design with four sheep (50.2$\pm$1.6 kg body weight). Sheep were allotted in one of four treatments, which were designed to progressively substitute Eucommia ulmoides for 0, 3, 5 and 10% of rice straw in the basal diet. The digestibility of crude fat was significantly (p<0.05) improved by 12.5 to 17.5% in 3% and 5% air dried Eucommia ulmoides leaves compared with that in control. The digestibility of crude fiber in the 3, 5 and 10% treatments was significantly (p<0.05) higher than that of the control. Dry matter intake was highest (p<0.05) in sheep fed 10% air dried E. ulmoides). Retained nitrogen in the 3, 5 and 10% treatments was increased up to 97%, 173% and 192%, respectively, compared to that in control (p<0.05). Organic matter utilization was higer in 3% and 5% treatments than control (p<0.05). Purine derivatives and microbial nitrogen synthesis were significantly increased by the dietary substitution of 5 % E. ulmoides leaves (p<0.05) compared with control and the 3% treatment. Ruminal concentration of total-VFA was significantly (p<0.05) higher in sheep fed all levels of air dried Eucommia ulmoides than those of control. It is concluded that air-dried E. ulmoides leaves given to ruminants can increase nutrient digestibility and palatability and improve ruminal fermentation. Therefore, the 5 and 10% substitution of E. ulmoides for roughage are highly recommended to be used in practice.


Eucommia ulmoides;Dry matter intake;Digestibility;Ruminal fermentation;Microbial nitrogen


  1. AOAC. 1990. Official method of analysis, Association of Official Agricultural Chemists. Washington, D. C. USA
  2. Bergmer, H. U. 1974. Methods of enzymatic analysis. Academic Press. New York. USA. p. 84
  3. Chen, X. B. and Gomes, M. J. 1995. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives : An overview of the technical details. Rowett Res. Ins
  4. Deyama, T., Ikawa, T., Kitagawa, S. and Nishibe, S. 1987. The constituents of Eucommia ulmoides Oliv VI. Isolation of a new sesquilignan and neolignam glycoside. Chem. Pharm. Bull. 35:1803
  5. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11: 1
  6. Fenner, H and Elliot, J. M 1963. Quantitative method for determining the steam volatile fatty acid in the rumen fluid by gas chromatography. J. Anim. Sci. 22:624
  7. Hoover, W. and Stokes, S. 1991. Balancing carbohydrates and proteins for optimum rumen microbial yield. J. Dairy Sci. 74:3630
  8. Hu, S. Y. A. 1978. Contribution to our knowledge of Tu-Chung-Eucommia ulmoides, American J. Chinese Med. 7:5
  9. Hwang, W. K., Choi, S. B. and Kim, I. H. 1996. Physiological activities of mixed extracts of Acantopancis senticosi radicis cortex and Eucommiae cortex. Kor J. Pharmacogn. 27:65
  10. Kim, J. H, Yokota, H., Ko, Y. D., Okajima, T. and Ohshima, M. 1993. Nutritional quality of whole crop com forage ensiled with cage layer manure. II. In situ degradability and fermentation characteristics in the rumen of goats. Asian-Aus. J. Anim. Sci. 6:53
  11. Kimura, Y., Okuda, H., Okuda, T., Hatano, T., Agata, I. and Arichi, S. 1985. Studies on the activities of tannins and related compounds from medicinal plants and drug. VII. Effects of extracts of leaves of Artemisia, species and caffeic acid and chlorogenic acid on lipid metabolic injury in rats fed peroxidixed oil. Chem. Pharm. Bull. 33:2028
  12. Leng, R. A. 1989. In ruminant physiology and nutrition in Asia(Eds. C. Devendra and E. Imaizumi). Jap. Soc. of Zootech. Sci
  13. Metroi, K, Furutsu, M. and Takahashi. S. 1997. The prevention effect of ginseng with Du-Zhung leaf on protein metabolism in aging. Biol. Pharm. Bull., 20:237
  14. Nakasa, T., Yamaguchi, M., Okinaka, O. and Metori, K. 1995. Effect of Du-chung leaf extract on plasma and hepatic lipids in rats fed on a high fat plus high cholesterol diet. Nippon Nogeikagaku Kaishi. 69:1491
  15. Oltjen, R. R., Slyter, L. L., Kozak, A. S. and Williams, E. E. jr. 1968. Evaluation of urea, biuret, urea phosphate and uric acid as NPN sources for cattle. J. Nutr. 94: 193
  16. SAS. 1990. SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, NC, U.S.A
  17. Satter, L. D. and Slyter, L. L. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutr. 32:199
  18. Siddons, R. C. Paradine, J., Gale, D. L. and Evans, R. T. 1985. Estimation of the degradability of dietary protein in the sheep rumen by in vivo and in vitro procedures. Br. J. Nutr. 54:545
  19. Sniffen, C. J. and Robinson, P. H. 1987. Microbial growth and flow as influenced by dietary manipulations. J. Dairy Sci. 70:425
  20. Stokes, S. R, Hoover, W. H., Miller, T. K. and Manski, R. P. 1991. Impact of carbohydrate and protein levels on bacterial metabolism in continuous culture. J. Dairy Sci. 74:860
  21. Tharib, S. M., Gnan, S. O. and Veitch, G. D. A. 1983. Antimicrobial activity of compounds from Artemisia campestris. J. Food Protection. 46: 185
  22. Van Soest, P. J., Roberts, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74: 3583
  23. 森本. 1971. 動物營養實驗法. 養賢堂. 日本
  24. 김영민. 2005. 두충의 반추동물 사료개발에 관한 연구. 경상대학교 박사학위논문
  25. 맹원재. 1998. 반추동물영양학 : 반추위 미생물과 소화작용. 향문사. p 128
  26. 박성진, 김만배. 1996. 두충잎의 첨가가 육계의 성장 및 육질에 미치는 영향. 한국가금학회지. 23:71