Activation of RAW 264.7 Macrophage by Digested Bacterial Cell of Pig-derived Lactobacillus Strains

돼지에서 분리된 Lactobacillus Strains의 균체분해산물에 의한 RAW 264.7 Macrophage 활성화

  • Kim, D.W. (National Livestock Research Institute, RDA) ;
  • Cho, S.B. (National Livestock Research Institute, RDA) ;
  • Jeong, H.Y. (National Livestock Research Institute, RDA) ;
  • Moon, H.G. (National Livestock Research Institute, RDA) ;
  • Lee, H.J. (National Livestock Research Institute, RDA) ;
  • HwangBo, J. (National Livestock Research Institute, RDA) ;
  • Chung, W.T. (National Livestock Research Institute, RDA) ;
  • Choi, C.W. (National Livestock Research Institute, RDA) ;
  • Chung, I.B. (National Livestock Research Institute, RDA)
  • 김동운 (농촌진흥청 축산연구소) ;
  • 조성백 (농촌진흥청 축산연구소) ;
  • 정하연 (농촌진흥청 축산연구소) ;
  • 문홍길 (농촌진흥청 축산연구소) ;
  • 이현정 (농촌진흥청 축산연구소) ;
  • 황보종 (농촌진흥청 축산연구소) ;
  • 정완태 (농촌진흥청 축산연구소) ;
  • 최창원 (농촌진흥청 축산연구소) ;
  • 정일병 (농촌진흥청 축산연구소)
  • Published : 2005.12.31


This study was conducted to investigate the effects of hydrolyzed Lactobacillus supplementation with digestive enzymes treatment on the macrophage activation, the induction of nitric oxide(NO), interleukin (IL)-6 and tumor necrosis factor(TNF)-$\alpha$. The RAW 264.7 murine macrophage was exposed to porcine Lactobacillus strains which were digested with both pepsin and pancreatin. The production of NO, TNF-$\alpha$ and IL-6 in the macrophage were strain and dose-dependent, respectively. The induction of NO and cytokines were higher in both 3149 and 3146 strains compared with other Lactobacillus strains. Overall, the level of NO was observed at the lower range between 10 and 150 μg hydrolysates per ml, whereas IL-6 and TNF-$\alpha$ were observed at relatively higher concentration between 50 and 300 μg hydrolysates per ml. Lactobacillus strains which produced a high level of NO also showed a high induction of TNF-$\alpha$ and IL-6. Therefore, the present results suggest that hydrolysates of Lactobacillus strains are related to induction of several macrophage mediators, and then it could be beneficially used to modulate gastrointestinal immune function of the host. Also, the methodogly employed in this study might be useful to investigate the effects of lactic acid bacteria on gastrointestinal immunity.


Lactobacillus;Macrophage;Nitric oxide;Cytokine


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