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Studies on Frozen Semen Characteristics Following Pentoxifylline Treatment and Artificial Insemination in Dog

개에서 Pentoxifylline 첨가에 따른 동결정액 성상과 인공수정에 관한 연구

  • Ji, D.Y. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Kim, C.K. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Lee, J.H. (National Livestock Research Institute, R.D.A.) ;
  • Park, S.J. (National Livestock Research Institute, R.D.A.) ;
  • Ryu, L.S. (National Livestock Research Institute, R.D.A.) ;
  • Ryu, J.W. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Lee, J.H. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Jeong, Y.C. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University) ;
  • Pang, M.G. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
  • 지달영 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 김창근 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 이장희 (농촌진흥청 축산연구소) ;
  • 박상재 (농촌진흥청 축산연구소) ;
  • 류일선 (농촌진흥청 축산연구소) ;
  • 류재원 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 이주형 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 정영채 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원) ;
  • 방명걸 (중앙대학교 동물자원과학과, 중앙대학교 생명환경연구원)
  • Published : 2005.12.31

Abstract

The present study evaluated whether pentoxifylline added to the freezing extender could improve the sperm characteristics and function in canine frozen semen. Also the conception rate following AI with frozen-thawed semen was investigated. The beneficial effects of pentoxifylline supplementation were visible in motility, viability, acrosome reaction, and tail swelling patterns. Especially, highest sperm viability and function were obtained in the forzen semen supplemented with 1mM pentoxifylline. The follicle size measured by ultrasonography was 6.48 mm, 11.52 mm and 8.9 mm on 11, 13 and 15 days after the onset of natural estrus, respectively and ovulation occurred on 13 and 15 days. The pregnancy rates in bitches inseminated with frozen semen on natural and induced estrus were 71.4% and 75.0%, respectively. There was no significant difference between the pregnancy rates in bitches inseminated with frozen semen following natural and induced estrus, but the litter size was slightly increased in natural cycle.

Keywords

Pentoxifylline;Dog;CASA;Frozen Semen;AI

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