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Differentiation of Hanwoo Intramuscular Preadipocytes

한우 Intramuscular Preadipocyte의 분화

  • Lee, S.M. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University) ;
  • Jeong, Y.H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University) ;
  • Hwang, S.H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University) ;
  • Park, H.Y. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Moon, S.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University) ;
  • Chung, E.R. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University) ;
  • Kang, M.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science Chonnam National University)
  • 이상미 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 정영희 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 황성호 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 박효영 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 윤두학 (농촌진흥청 축산연구소) ;
  • 문승주 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 정의룡 (상지대학교 생명자원과학대학 생명공학과) ;
  • 강만종 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부)
  • Published : 2005.12.31

Abstract

The development of marbling in cattle is closely associated with an increase in adipocyte size and number within muscle. The adipose precursor cells have the capacity to differentiate into adipocytes within the muscle during the formation of marbling. In this studies, we established the cell culture system for differentiation of intramuscular preadipocyte isolated from the sirloin of Hanwoo aged 12 months. The intramuscular preadipocyte cells exhibited a fibroblastic appearance and differentiated into adipocytes by treating confluent cells with differentiation medium containing insulin, dexamethasone, and troglitazone. When intramuscular preadipocyte cells were differentiated at 18 day, the triglyceride concentration was higher than control cells. Moreover, the thiazolidinedione treatment increased adipogenesis. RT-PCR analysis confirmed the significant expression of PPARγ mRNA during adipocyte differentiation. In conclution, our culture system used in this study allowed intramuscular preadipocyte cells to differentiated into adipocytes and intramuscular preadipocyte cells may be useful in the further study of differentiation mechanism of adipocytes in Hanwoo.

Keywords

Primary culture;Bovine;Thiazolidinedione;Peroxisom proliferator-activated receptor-${\gamma}$;Preadipocyte

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