Stability of Anti-Helicobacter pylori IgY Under Some Condition

Anti-Helicobacter pylori IgY의 처리조건에 따른 안정성에 관한 연구

  • Published : 2005.10.31


The aim of this experiment was to evaluate the stability of anti-Helicobacter pylori IgY in water soluble fraction(WSF) of egg yolk according to the heat, pH and digestive enzyme treatment. Anti-H. pylori IgY content of WSF remained 76% after pasteurization(63$^{\circ}C$ for 30 min). The stability of anti-H. pylori IgY at different pH showed a tendency to diminish according to decreasing pH from 7.0 to 1.5(p<0.05). Anti-H. pylori IgY content was 84.4% after treatment for 1 hour at 37$^{\circ}C$ in pH 5.0. There were significantly differences in IgY content between 1 hour and 2 hours at pH 2.0 in 200 units of pepsin treatment(p<0.05). However, IgY was relatively stable at pH 4.0 regardless of the reaction time and the concentration of pepsin. The stability of IgY of egg yolk after the treatment of trypsin was significantly higher than that of water soluble fraction (p<0.05). This results indicated that anti-H. pylori IgY showed relatively a good stability on heat, pH and digestive enzyme.


  1. Hunt, R. H. 1996. Eradication of Helicobacter pylori infection. Am. J. Med. 100(5A):42S
  2. Brenner, H., Rothenbacher, D., Bode, G. and Adler, G. 1999. Inverse graded relation between alcohol consumption and active infection with Helicobacter pylori. Am. J. Epidemiol. 149:571
  3. Chen, C. C., Tu, Y. Y. and Chang, H. M. 1999. Efficiency and protective effect of encapsulation of milk immunoglobulin G in multiple emulsion. J. Agric. Food Chem. 47:407
  4. Correa, P., Fontham, E. T., Bravo, J. C., Bravo, L. E., Ruiz, B., Zarama, G., Realpe, J. L., Malcom, G. T., Li, D., Johnson, W. D. and Mera, R. 2000. Chemoprevention of gastric dysplasia: randomized trial of antioxidant supplements and anti-Helicobacter pylori therapy. J. Natl. Cancer Inst. 92:1881
  5. Hopkins, R. J., Girardi, L. S. and Turney, E. A. 1996. Relationship between Helicobacter pylori eradication and reduced duodenal and gastric ulcer recurrence. Gastroenterology. 110:1244
  6. Lee, K. A., Chang, S. K., Lee, Y. J., Lee, J. H. and Koo, N. S. 2002. Acid stability of anti- Helicobacter pyroli IgY in aqueous polyol solution. J. Biochem. Mol. Biol. 35:488
  7. Mabe, K., Yamada, M., Oguni, I. and Takahashi, T. 1999. In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob. Agents Chemother. 43:1788
  8. NIH(National Institute of Health) Consensus Con- ference. 1994. Helicobacter pylori in peptic ulcer disease. JAMA. 272:65
  9. Owen, R. J. 1998. Helicobacter-species classification and identification. Br. Med. Bull. 54:17
  10. Parsonnet, J., Friedman, G. D., Vandersteen, D. P., Chang, Y., Vogelman, J. H., Orentreich, N. and Sibley, R. K. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325:1127
  11. Parsonnet, J., Hansen, S., Rodriguez, L., Gelb, A. B., Warnke, R. A., Jellum, E., Orentreich, N., Vogelman, J. H. and Friedman, G. D. 1994. Helicobacter pylori infection and gastric lymphoma. N. Engl. J. Med. 330:1267
  12. Patterson, R., Youngner, J. S., Weigle, W. O. and Dixon, F. J. 1962. Antibody production and transfer to egg yolk in chickens. J. Immunol. 89:272-278
  13. Peterson, W. L. 1991. Helicobacter pylori and peptic ulcer disease. N. Engl. J. Med. 324:1043
  14. Warren, J. R. and Marshall, B. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1:1273
  15. 박민정, 김주성, 임정윤, 정현채, 송인성, 유은실, 이정준, 허철성, 백영진. 2001. Lactobacillus가 함유된 발효유에 의한 인체 위점막에서의 Helicobacter pylori 억제효과. 대한소화기학회. 38:233
  16. 손동화, 노정해, 김영붕, 한찬규, 성기승, 이남형. 1998. 난황으로 부터 항충치 항체의 분리 및 그 특성. 한국식품과학회. 30:1029
  17. 이광호, 윤희상, 백승철, 이우곤, 조명제, 최휴진, 맹국영, 고광욱. 1990. 한국인(韓國人)의 위염(胃炎) 원인균(原因菌) Helicobacter pylori 보균실태(保菌實態). 대한미생물학회. 25:475