Antioxidant Activity and Characterization of Exiguobacterium sp. SC2-1 Isolated from Sea Water

해양에서 분리한 Exiguobacterium sp. SC2-1의 항산화 활성 및 특성

  • Kim Man-Chul (Faculty of Applied Marine Science, Cheju National University) ;
  • Park Guen- Tae (Faculty of Applied Marine Science, Cheju National University) ;
  • Son Hong-Joo (Dept. of Biotechology, Miryang National University) ;
  • Choi WooBong (Dept. of Biotechology & Bioengineering, Dongeui University) ;
  • Heo Moon-Soo (Faculty of Applied Marine Science, Cheju National University)
  • 김만철 (제주대학교 해양과학대학 해양생물공학과) ;
  • 박근태 (제주대학교 해양과학대학 해양생물공학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 최우봉 (동의대학교 생물공학과/바이오물질제어학과) ;
  • 허문수 (제주대학교 해양과학대학 해양생물공학과)
  • Published : 2005.03.01

Abstract

For the reseach of the natural marine antioxidant, an antioxidant-producing bacterium was isolated from seawater in Jeju costal area. The isolated strain SC2-1 was Gram-positive, catalase positive, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and NaCl required for growth. The radical scavenging activity of the culture supernatant was detemined by DPPH method. This bacterium was identified based on morphological, biochemical characteristics and 16S rDNA sequence analysis, and then was named Exiguobacterium sp. SC2-1. The optimum conditions of culture for production antioxidnat were $25^{\circ}C$, pH 6-8 and $4\%$ NaCl. The stain showed the highest activity and growth cultured in medium which added $1\%$ maltose. Hydroxyl radical activity of the supernatant of Exiguobacterium sp. SC2-1 was $73\%$. The SOD activity of the culture supernatant was estimated about $35\%$.

Keywords

DPPH;Exiguobacterium sp.;hydroxyl radical;SOD

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