Identification of Bacterial Strains Adhered to Human Scalp Hair and Antimicrobial Susceptibility

사람 모발에 부착되어 있는 세균의 동정 및 항생제 감수성

  • Lee Moon Sook (Department of Biology, Sunchon National University) ;
  • Han Hyo Shim (Department of Biology, Sunchon National University) ;
  • Jung Jae Sung (Department of Biology, Sunchon National University)
  • Published : 2005.03.01


This study was carried out to identify bacterial strains adhered to human scalp hair and to investigate the antibiotic susceptibility of them. A total of 39 isolates were obtained from patients in intensive care units and healthy persons. The most common species isolated was Staphylococcus epidermidis (19 isolates), followed by S. aureus (14 isolates), S. waneri (5 isolates), and S. pasteuri (1 isolate). The susceptibility of isolates to amikacin, ampicillin, bacitracin, carbenicillin, cefazolin, cefoperazone, chloramphenicol, erythromycin, gentamicin, methicillin, nalidixic acid, neomycin, oxacillin, penicillin, streptomycin, tetracycline and vancomycin was determined by the disk diffusion method. All of the antibiotic resistant isolates were obtained from patient scalp hair. To examine the effect of conventional shampoo and detergent SDS on removing of bacteria from hair, we treated hair with culture solution of S. aureus. The bacteria attached to hair were not removed even by repeated washing with detergents. These results suggested that hair could be a source of bacterial contamination in hospital.


antimicrobial susceptibility;cross-contamination;human scalp hair;Staphylococcus


  1.  Angeliki, P., J. Paparaskevas, S. Ioanna, and A. Athina. 2003. Changes in the epidemiology of methicillin-resistant Staphylococcus aureus in a Greek teriary care hospital, over an 8-year-period. Int. J. Antimicrob. Agents. 21, 542-546
  2.  Assadian, O., F. Daxboeck, C. Aspeck, A. Blacky, R. Dunkl, and W. Koller. 2003. National surveillance of methicillin-sensitive and methcillin-resistant Staphylococcus aureus in Austrian hospitals: 1994-1998. J. Hosp. Infect. 55, 175-179
  3. Beasley, W.D., and G. Hirst. 2003. Making a meal of MRSA-the role of biosurgery in hospital-acquired infection. J. Hosp. Infect.55, 454-459
  4. Boyce, J.M., G. Potter-Bynoe, C. Chenevert, and T. King. 1997. Environmental contamination due to methicillin-resistant Staphylococcus aureus: Possible infection control implications. Epidemiol. 18, 622-627
  5.  Chambers H.F. 1997. Methicillin resistance in Staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10, 781-791
  6.  Huijsmans-Evers, A.G.M. 1978. Results of routine tests for the detection of dispersers of Staphylococcus aureus. Arch. Chir. Neerl. 30, 141-150
  7.  Kloos, W.E., and T.L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7, 117-140
  8.  Leclercq, R., C.J. Soussay, P. Weber, and N. Moniot-Ville. 2003. In vitro activity of the pristinamycin against the isolated staphylococci in the French hospitals in 1999-2000. Pathol. Biol. 51, 400-404
  9. Pfaller, M.A., and L.A. Herwaldt. 1998. Laboratory, clinical and epidemiological aspects of coagulase-negative staphylococci. Clin. Microbiol. Rev. 1, 281-299
  10.  Shiomori, T., H., Miyamoto, and K. Makishima. 2001, Significance of airborne transmission of methicillin-resistant Staphylococcus aureus in an otolaryngology head and neck surgery unit. Arch. Otolaryngol. Head Neck Surg. 127, 644-648
  11. Takashi, K. 2003. Survey of methicillin-resistant coagulase negative staphylococci isolated from the fingers of nursing students. J. Infect. Chemother. 9, 30-34
  12. Teixeira, L.A., C.A. Resende, and L.R. Ormonde. 1995. Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J. Clin. Microbiol. 33, 2400-2404
  13.  Wang, J.T., Y.C. Chen, T.L. Yang, and S.C. Chang. 2002. Molecular epidemiology and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus in Taiwan. Diagnostic Microbiol. Infect. Dis. 42, 199-203
  14.  Han, H.S., Y.J. Koh, J.-S. Hur, and J.S. Jung. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13, 110-118
  15.  Dominguez, M.A., H. Lencastre, J. Linares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin resistant Staphylococcus aures (MRSA) clone during an outbreak of MRSA disease in a Spainish Hospital. J. Clin. Microbiol. 32, 2081-2087
  16. Marrack, P., and J. Kappler. 1990. The staphylococcal enterotoxins and their relatives. Science 248, 705-711
  17.  Berger-Bachi B. 1997. Resistance not mediated by $\beta$-lactamase (methicillin resistance). p158-167. In Crossley K. B., Archer G. L., eds. The staphylococci in human disease. New York, Churchill Livingstone
  18. Ruddy, M., M. Cummins, and Y. Drabu. 2001. Hospital hairdresser as a potential source of cross-infection with MRSA. J. Hosp. Infect. 49, 225-227
  19.  Deplano, A., A. Schuermans, J. Eldere, W. Witte, H. Meugnier, and J. Etienne. 2000. Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive element PCR analysis. J. Clin. Microbiol. 38, 3527-3533
  20.  Park, C.H., K.J. Choi, S.J. Moon, and C.S. Uhm. 2002. Structural development of scalp hair in children and effects of cosmetologic physico-chemical treatments on adult scalp hair. Kor. J. Electron. Microscop. 32, 339-344
  21. McDonald, P., E. Mitchell, H. Johnson, and R. McDonnell. 2003. Epidemiology of MRSA: the North/South study of MRSA in Ireland 1999. J. Hosp. Infect. 54, 130-134
  22. Park, S.G., Y.O. Hwang, J.H. Jang, and K.M. Lee. 2001. Biological characteristics of Staphylococcus aureus isolated from food-born patients in Seoul. J. Food Hyg. Safety 16, 159-167
  23.  Silva-Coimbra, M.V., M.C. Silva-Carvalho, H. Wisplinghoff, G.O. Hall, and R.P. Wenzel. 2003. Clonal spread of methicillin resistant Staphylococcus aureus in a large geographic area of the United States. J. Hosp. Infect. 253, 103-110
  24.  Bradley S.F. 1992. Methicillin-resistant Staphylococcus aureusinfection. Clin. Geriatr. Med. 8, 853-868
  25.  Nakamura, R., N. Misawa, T. Oguri, and J. Igari. 1997. Incidence of coagulase negative staphylococci isolated from clinical materials, and drug resistance of isolates. J. Jpn. Clin. Microbiol. 7, 101-110
  26.  Moyer, C.L., F.C. Dobbs, and D.M. Karl. 1994. Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loihi Seamount, Hawaii. Appl. Environ. Microbiol. 60, 871-879
  27. Duckworth, G., B. Cookson, and H. Humphreys. 1998. Revised guidelines for the control of methicillin-resistant Staphylococcus aureus infection in hospitals. J. Hosp. Infect. 39, 253-290
  28.  Lizioli, A., G. Privitera, E. Alliata, E.M. Antonietta., and V. Carreri. 2003. Prevalence of nosocomial infection in Italy: result from the Lombardy survey in 2000. J. Hosp. Infect. 54, 114-148
  29.  Kiyomi, M., H. Toshinobu, H. Kazuto, K. Yasushi, Y. Takafumi, and O. Michio, 2000. Firm adherence of Staphylococcus aureusand Staphylococcus epidermidis to human hair and effect of detergent treatment. Microbiol. Immunol. 44, 453-656
  30. Timothy, J.F., and H. Magnus. 1998. Surface protein adhesins of Staphylococcus aureus. Trend. Microbiol. 6, 484-488
  31. Takashi, I., R. Okamoto, C. Shimauchi, T. Okubo, A. Kuga, and M. Inoue. 2001. Identification of aminoglycoside-modifying enzymes by susceptibility testing: Epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J. Clin. Microbiol. 39, 3115-3121
  32. Foster, T.J., and M. Hook. 1998. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 6, 484-8
  33.  Patti, J.M., B.L. Allen, M.J. McGavin, and M. Hook. 1994. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 45, 585-617
  34. Patrick, C.C. 1990. Coagulase-negative staphylococci: pathogens with increasing clinical significance. J. Pediatrics 116, 497-507