A spsB Gene Putatively Encoding Glucosyl-Isopreny Phosphate-Transferase in Sphingomonas chungbukensis DJ77

Sphingomonas chungbukensis DJ77의 Glucosyl-Isoprenyl Phosphate-Transferase를 암호화할 것으로 추정되는 spsB 유런자

  • Lee Soo-Youn (School of Life Science, Chungbuk National University) ;
  • Choi Jung-Do (School of Life Science, Chungbuk National University) ;
  • Shin Malshick (Department of Food and Nutrition, Chonnam National University) ;
  • Kim Young-Chang (School of Life Science, Chungbuk National University, Biotechnology Research Institute, Chungbuk National University)
  • 이수연 (충북대학교 자연과학대학 생명과학부) ;
  • 최정도 (충북대학교 자연과학대학 생명과학부) ;
  • 신말식 (전남대학교 생활과학대학 식품영양학과) ;
  • 김영창 (충북대학교 자연과학대학 생명과학부, 충북대 바이오 연구소)
  • Published : 2005.03.01

Abstract

Some genes, which are involved in the biosynthesis of polysaccharides, could be found by the genome project of Sphingomonas chungbukensis DJ77. In this study, we identified the complete nucleotide sequence of a gene, encoding the glucosyl-isoprenyl phosphate-transferase, which catalyzes the first step in the biochemical pathway for the synthesis of the sphingan type polysaccharide. This gene, named spsB, is initiated by the ATG codon and terminated by the TGA, and its open reading frame consists of 1392 bp, encoding 463 amino acids. The predicted amino acid sequence of this enzyme indicates $50\%$ similarity to SpsB of Sphingomonas spp S88, also produces sphingan, and $48\%$ to GelB of Sphingomonas paucimobilis ATCC 31461.

Keywords

glucosyl-isoprenyl phosphate-transferase;Sphingomonas chungbukensis DJ77;spsB

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