Cloning and Structural Analysis of bfmo Operon in Methylophaga aminosulfidovorans SK1

Methylophaga aminosulfidovorans SKI bfmo 오페론의 클로닝 및 구조 분석

  • Lim Hyun Sook (Graduate School of Education, Chosun University) ;
  • Goo Jae Whan (Department of Environmental engineering, Chosun University) ;
  • Kim Lee Hyun (Graduate School of Education, Chosun University) ;
  • Kim Si Wouk (Department of Environmental engineering, Chosun University) ;
  • Cho Eun Hee (Department of Biology Education, Chosun University)
  • Published : 2005.03.01

Abstract

Methylophaga aminosulfidovorans SK1 (KCTC 10323 BP) can utilize trimethylamine as a sole carbon, nitrogen, and energy source. The bacterial flavin-containing monooxygenase (bFMO) gene was identified in the strain and the recombinant enzyme expressed in E. coli oxidized trimethylamine. To study the function and regulation of the bfmo, over 8,000 nucleotide sequences of the neighboring regions including the bfmo were determined. Three open reading frames proceeding to the bfmo gene encoded analogues to highly conserved nitrate/nitrite sensing two-component system regulators and a methyl accepting protein. Two small open reading frames just downstream of the bfmo gene showed no similar proteins of known functions but the sequences were conserved among other bacteria. Reverse transcription-polymerase chain reaction analysis showed that the six putative genes consisted of three transcription units. The three regulatory genes located upstream of the bfmo gene formed two separate transcription units. The bfmo and the two downstream genes were transcribed from a single promoter.

Keywords

bacterial operon;flavin-containing monooxygenases(FMO);Methylophaga;methylotrophs;RT-PCR

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