Determination of Soil Nitrogen Supplying Capacity Using Pepsin Digestibility

Pepsin 분해방법을 이용한 토양의 질소 공급력 결정

  • Kim, Yoo-Hak (National Institute of Agricultural Science and Technology) ;
  • Kim, Sun-Kwan (National Institute of Agricultural Science and Technology) ;
  • Zhang, Yong-Sun (National Institute of Agricultural Science and Technology)
  • Received : 2005.09.01
  • Accepted : 2005.10.07
  • Published : 2005.10.30

Abstract

It is necessary to determine a nitrogen supplying capacity (NSC) of soil for sustainable agriculture. NSC has been decided by directly detecting N mineralization potential (NMP) and inorganic nitrogen or by indirectly approximating from organic matter and chemical properties of soil. NMP is best method for NSC but it takes long period. A study was conducted to find a short-term incubation method using pepsin through 1) determining NMP of 3 upland and 3 paddy soils, 2) establishing analytical condition of pepsin digestion by comparing to NMP, 3) validating with relations to N requirements for maximum yield of rice. NMPs of 6 soils were ranges from $63mg\;N\;kg^{-1}$ to $156mg\;N\;kg^{-1}$. The pepsin digestion method of soil nitrogen was established by determining amino nitrogen from digesting 5 g of soil for 30 minutes by 0.02% pepsin. This method was so highly correlated with a maximum rate of nitrogen fertilizer that it could be used for determining NSC in paddy soil.

Keywords

Pepsin digestibility;Nitrogen supplying capacity;Nitrogen mineralization potential

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