Effects of Haeganjeon on Immobilization-Stress or Cold-Stress in Mice

해간전(解肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響)

  • Hwang, Tae-Won (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang university) ;
  • Sim, Ho-Cherl (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang university) ;
  • Kim, Song-Baeg (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang university) ;
  • Yoo, Sim-Keun (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang university) ;
  • Cho, Han-Back (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang university)
  • 황태원 (원광대학교 한의과대학 부인과학교실) ;
  • 심호철 (원광대학교 한의과대학 부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 부인과학교실) ;
  • 유심근 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실)
  • Published : 2005.08.31

Abstract

Purpose : Investigate the effects of Haeganjeon water extract (HGJ) on immobilization-stress or cold-stress in C57BL/6J mice. Methods : Male C57BL/6J 30 mice of weighting 18${\pm}$2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress HGJ oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress HGJ oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system. Results : HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells. In addition, HGJ enhanced the cell viability of thymocytes decreased by immobilization-stress or cold-stress and decreased DNA fragmentation of thymocytes increased by immobilization- stress or cold-stress. Also, HGJ increased the cell viability of splenocytes decreased by cold-stress and decreased DNA fragmentation of splenocytes increased by cold-stress. HGJ decreased the population of thymic CD4+ cells increased by immobolization-stress. HGJ increased the population of B220+ cells decreased by immobilization-stress and decreased the population of Thy1+ cells increased by immobilization-stress. Also, HGJ decreased the population of splenic CD4+ cells increased by immobolization-stress. HGJ enhanced the production of ${\gamma}-interferon$ decreased by immobilization-stress or cold-stress and increased the production of interleukin-4 decreased by immobilization-stress. Furthermore, HGJ enhanced the phagocytic activity decreased by immobilization-stress or cold-stress and enhanced the level of nitric oxide decreased by immobilization-stress or cold-stress. Conclusion : HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.