In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant

  • Rajasekharan P.E. (In Vitro Conservation and Cryopreservation Laboratory, Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake) ;
  • Ambika S.R. (Physiology laboratory, Department of Botany, Bangalore University) ;
  • Ganeshan S. (In Vitro Conservation and Cryopreservation Laboratory, Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake)
  • Published : 2005.06.01

Abstract

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.

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