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Protective Effect of Acanthopanax senticosus Extract on Alloxan-induced β-cell Damage

  • Rho, Hye-Won (Department of Biochemistry, Medical School and Chonbuk National University, Institute of Cardiovascular Research, Chonbuk National University) ;
  • Lee, Ji-Hyun (Department of Biochemistry, Medical School and Chonbuk National University) ;
  • Kim, Jong-Suk (Department of Biochemistry, Medical School and Chonbuk National University) ;
  • Kim, Hyung-Rho (Department of Biochemistry, Medical School and Chonbuk National University) ;
  • Park, Byung-Hyun (Department of Biochemistry, Medical School and Chonbuk National University) ;
  • Park, Jin-Woo (Department of Biochemistry, Medical School and Chonbuk National University)
  • Published : 2005.03.01

Abstract

The protective effect of Acanthopanax senticosus (AS) extract on alloxan-induced pancreatic β-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic β-cell line. Alloxan caused the pancreatic β-cell damage through the generation of reactive oxygen free radicals, increased DNA fragmentation, and decreased cellular NAD/sup +/ levels. The β-cell damage was significantly prevented by the pretreatment with water soluble extract of AS roots. These results suggest that the protective effect of AS extract, on alloxan-induced β-cell damage, is primarily due to the inhibition of the generation of reactive oxygen free radical species (ROS) by alloxan.

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