Association between Microsatellite DNA Marker of Leptin Gene and Carcass Traits in Korean Cattle

  • Chung Eui-Ryong (Department of Biotechnology, Sangji University) ;
  • Chung Ku-Young (Department of Animal Science and Technology, Sangji University)
  • Published : 2005.03.01

Abstract

Leptin, the product of the obesity (ob) gene, is synthesized in adipocytes or fat cells and has been implicated in the regulation of food intake, energy balance and body composition in mammals. Therefore, the leptin gene could be a candidate gene controlling fat deposition, meat quality and carcass traits in cattle. In this study the microsatellite genotypes for leptin gene were determined and their effects on carcass traits and meat quality were estimated in Korean cattle. Six different microsatellite alleles within leptin gene were identified and gene frequencies of 173, 177, 184, 186, 190 and 192 bp alleles were 0.012, 0.308, 0.067, 0.260, 0.342 and 0.016, respectively. The microsatellite marker of the leptin gene showed a significant association with the carcass percentage (CP) and marbling score (MS). Animals with genotypes 192/192 and 177/184 had higher CP than animals with other genotypes. Animals with genotypes 184/192 and 177/184 had higher MS compared with animals with other genotypes. Thus, the results suggest that the 177, 184 and 192 bp alleles may be associated with increased carcass percentage and intramuscular fat levels. No associations were found between the microsatellite genotypes of the leptin gene and other carcass traits such as carcass weight (CW), backfat thickness (BF) and M. longissimus dorsi area (LDA). In conclusion, the microsatellite markers of the leptin gene may be useful for marker-assisted selection of carcass traits and meat quality in Korean cattle.

References

  1. Bassam, J. B., Caetano-Anolles, G., and Gresshof, P. M. (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196, 80-83 https://doi.org/10.1016/0003-2697(91)90120-I
  2. Fitzsimmons, C. J., Schmufz, S. M., Bergen, R. D., and Mckinnon, J. J. (1998) A potential association between the BM 1500 microsatellite and fat deposition in beef cattle. Mamm. Genome 9, 432-434 https://doi.org/10.1007/s003359900791
  3. Friedman, J. M. and Halaas, J. L. (1998) Leptin and the regulation of body weight in mammals. Nature 395, 763-770 https://doi.org/10.1038/27376
  4. Geary, T. W., McFadin, E. L., MacNeil, M. D., Grings, E. E., Short, R E., Funston, R. N., and Keisler, D. H. (2003) Leptin as a predictor of carcass composition in beef cattle. J. Anim. Sci. 81, 1-8
  5. Grindflek, E., Holzbauer, R., plastow, G., and Rothschild, M. F. (2002) Mapping and investigation of the porcine major insulin sensitive glucose transport (SLC2A41GLuT4) gene as a candidate gene for meat quality and carcass traits. J. Anim. Breed. Genet. 119, 65-68 https://doi.org/10.1046/j.1439-0388.2002.00318.x
  6. Hirano, J., Nakane, S., Mizoshita, K., Yamakuchi, H., Inoue-Murayama, M., Watanabe, T., Barendse, W., and Sugimoto, Y. (1996) Characterization of 42 highly polymorphic bovine microsatellite markers. Anim. Genet. 27, 365-368 https://doi.org/10.1111/j.1365-2052.1996.tb00980.x
  7. Hossner, K. L. (1998) Cellular, molecular and physiological aspects of leptin: potential application in animal production. Canadian J. Anim, Sci. 78, 463-472 https://doi.org/10.4141/A98-061
  8. Jiang, Z. H. and Gibson, J. P. (1999) Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm. Genome 10, 191-193 https://doi.org/10.1007/s003359900968
  9. Kennes, Y. M., Murphy, B. D., Pothier, F., and Palin, M. F. (2001) Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Anim. Genet. 32, 215-218 https://doi.org/10.1046/j.1365-2052.2001.00768.x
  10. Maffei, M., Halaas, J., Ravyssin, E., Pratley, R E., Lee, G. H., Zhang, Y., and Fridman, J. M. (1995) Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Medicine 1, 1155-1161 https://doi.org/10.1038/nm1195-1155
  11. Miller, S. A., Dykes, D. D., and Polesky, H. F. (1988) A simple salting out procedure for extracting DNA from human nucleoted cells. Nucleic Acids Res. 16, 1215-1220 https://doi.org/10.1093/nar/16.3.1215
  12. Minton, J. E., Bindel, D. J., Droullard, J. S., Titgemeyer, E. C., Grieger, D. M., and Hill, C. M. (1998) Serum leptin is associated with carcass traits in finishing cattle. J. Anim. Sci. (Suppl.) 76, 231-237
  13. Pomp, D., Zou, T., Clutter, A. C., and Barendse, W. (1997) Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J. Anim. Sci. 75, 1427-1433
  14. Rothschild, M. F. and Soller, M. (1997) Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe 8, 13-20
  15. Savell, J. W. and Cross, H. R. (1998) The role of fat in the palatability of beef, pork and lamb. In: Designing foods, animal product options in the marketplace 99, 345-355
  16. Stone, R. T., Kappes, S. M., and Beattie, C. W. (1996) Two polymorphic microsatellites within an 18Kb genomic clone containing the bovine ob gene. Anim. Genet. 27, Suppl. 2, 64 https://doi.org/10.1111/j.1365-2052.1996.tb01189.x
  17. Tellam, R. L. (1996) Bas taurus leptin (obese) gene, complete CDs, GenBank Accession No. U50365
  18. Tessanne, K., Hines, H. C., and Davis, M. E. (1998) Relationships of polymorphisms in the bovine leptin gene with differences in beef carcass traits. Research and Reviews; Beef and Sheep, special circular 170-199
  19. Tokuda, T., Matsui, T., Ito, J., Torill, S., and Yano, H. (2000) The changes in body weight and plasma metabolic levels during leptin injection are caused by the reduction of food intake in sheep. Animal Sci. 73, 343-348
  20. Wheeler, T. L., Cundift, L. V., and Koch, R. M. (1994) Effect of marbling degree on beef palatability in Bas taurus and Bas indicus cattle. J. Anim. Sci. 72, 3145-3151
  21. Wilkins, R. J. and Davey, H. W. (1997) A polymorphic microsatellite in the bovine leptin gene. Anim. Genet. 28, 376-383 https://doi.org/10.1111/j.1365-2052.1997.tb03278.x