The effects of transformation capacity on cometabolic degradation of trichloroethene (TCE) were evaluated using TCE-degrading actinomycetes pure and mixed culture under various culture conditions. The TCE transformation capacity of the actinomycetes enrichment culture in a batch test with phenol addition was 1.0 mg of TCE/mg of volatile suspended solids (VSS). The resting cell TCE transformation capacity of the actinomycetes pure culture cell was 0.75 mg TCE/mg VSS, which increased to 2.0 mg TCE/mg VSS when phenol was added as an external substrate. When the pure culture had an internal substrate in the form of poly-β-hydroxybutyrate (PHB) at 19% of the cell mass, the resting cell TCE transformation capacity increased from 0.47 to 0.6 mg TCE/mg VSS. The presence of PHB increased transformation capacity by 57%, whereas, the addition of phenol caused more than two fold increase in transformation capacity. The actinomycetes culture showed the highest transformation capacity.



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