DOI QR코드

DOI QR Code

Effects of Six Antibiotics on the Activity of the Photosynthetic Apparatus and Ammonium Uptake of Thallus of Porphyra yezoensis

  • Oh, Min-Hyuk (Department of Molecular Biology, Pusan National University) ;
  • Kang, Yun-Hee (Department of Marine Science, Pusan National University) ;
  • Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University) ;
  • Chung, Ik-Kyo (Department of Marine Science, Pusan National University)
  • Published : 2005.06.01

Abstract

The modern integrated fish-seaweed mariculture has been tested to reduce the environmental impacts of an intensive fed culture. To obtain the best seaweed bioremediation performance, the effects of therapeutants used for fish disease control on the selected seaweed species should be considered. As a selected seaweed, Porphyra yezoensis was tested with six commercial antibiotics including erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, doxycycline, pefloxacin, and amoxicillin trihydrate under the batch incubation at a photon flux density of 10 $\mu$mol ${\cdot}m^{-2}\;{\cdot}\;s^{-1}$ at 15$^{\circ}C$. Among the tested commercial antibiotics, erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, and doxycycline showed decreases in Fv/Fm, the photochemical efficiency of photosystem II, with a dose-dependant and time-dependant manner. From the quenching analysis of chlorophyll fluorescence, three differential patterns were observed in the antibiotics-treated Porphyra: (1) high nonphotochemical quenching (NPQ) and low photochemical quenching (qP) in the cases of Erythromycin thiocyanate_B and amoxicillin trihydrate, (2) high NPQ and high qP in the case of pefloxacin and (3) low NPQ and low qP in the case of oxytetracycline. These results indicated that antibiotics affected in various ways on the photosynthetic apparatus, reflecting differential lesion sites of antibiotics. In addition, the rates of ammonium uptake also decreased with a decrease of Fv/Fm in P. yezoensis thalli treated with erythromycin thiocyanate_B and oxytetracycline. Therefore, the four antibiotics mentioned could affect the bioremediation capacity of the selected seaweed species in the integrated fish-seaweed mariculture system due to the decrease of photosynthetic activity and the simultaneous decrease of ammonium uptake.

References

  1. Black K.D. 2001. Environmental Impacts of aquaculture. Scheffield Academic Press, Sheffield
  2. Chopin T., Buschmann A.H., Halling C., Troell M, Kautsky N., Neffus A., Kraemer G.P., Zertuche-Gonzalez J.A., Yarish C. and Neefus C. 2001. Integrated seaweeds into marine aquaculture systems: a key toward sustainability. J. Phycol. 37: 975-986 https://doi.org/10.1046/j.1529-8817.2001.01137.x
  3. Dy D.T. and Yap H.T. 2001. Surge ammonium uptake of the culture seaweed, Kappaphycus alvarezii (Doty) Doty (Rhodophyra: Gigartinalse). J. Exp. Mar. Biol. Ecol. 265: 89-100 https://doi.org/10.1016/S0022-0981(01)00325-2
  4. Eu Y.-J., Ha, S.-B. and Lee C.-H. 1996. Effects of chilling injury in the light on chlorophyll fluorescence and D1 protein turnover in cucumber and pea leaves. J. Biochem. Mol. Biol. 29: 398-404
  5. Parsons T.M., Maita Y. and Lalli C.M. 1984. A manual for chemical and biological methods for seawater analysis. Pergamon Press, New York
  6. Rohacek K. 2002. Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships. Photosynthetica 40: 13-29 https://doi.org/10.1023/A:1020125719386
  7. Schreiber U., Bilger W. and Neubauer C. 1994. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E.D. and Caldwell M.M. (eds), Ecophysiology of Photosynthesis. Springer-Verlag, Berlin, pp 49-70
  8. Wu R.S.S. 1995. The environmental impacts of marine fish culture: towards a sustainable future. Mar. Pollut. Bull. 31: 159-166 https://doi.org/10.1016/0025-326X(95)00100-2
  9. Young E.B. and Beardall J. 2003. Papid ammonium- and nitrate-induced pertubationsd to Chl a fluorescence in nitrogen-streessed Dunaliella tertiolecta (Chlorophyta). J. Phycol. 39: 332-342 https://doi.org/10.1046/j.1529-8817.2003.02109.x

Cited by

  1. Streptomycin affects the growth and photochemical activity of the alga Chlorella vulgaris vol.132, 2016, https://doi.org/10.1016/j.ecoenv.2016.06.019
  2. Morpho-physiological response of Populus alba to erythromycin: A timeline of the health status of the plant vol.569-570, 2016, https://doi.org/10.1016/j.scitotenv.2016.06.152