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The study on the Fluorescence Characteristics of Several Freshwater Bloom Forming Algal Species and Its Application

수종 담수적조 원인종들의 형광특성과 적용연구

  • Son, Moon-Ho (Department of Environmental Science and Engineering, Inje University) ;
  • Zulfugarov, Ismayil S. (Department of Molecular Biology, Pusan National University) ;
  • Kwon, O-Seob (Department of Environmental Science and Engineering, Inje University) ;
  • Moon, Byoung-Young (School of Biotechnology and Biomedical Science, Inje Univesity) ;
  • Chung, Ik-Kyo (Department of Marine Science, Pusan National University) ;
  • Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University) ;
  • Lee, Jin-Ae (Department of Environmental Science and Engineering, Inje University)
  • 손문호 (인제대학교 환경학과) ;
  • ;
  • 권오섭 (인제대학교 환경학과) ;
  • 문병용 (인제대학교 생명공학부) ;
  • 정익교 (부산대학교 해양과학과) ;
  • 이춘환 (부산대학교 분자생물학과) ;
  • 이진애 (인제대학교 환경학과)
  • Published : 2005.06.01

Abstract

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

References

  1. Holm-Hansen O., Lorenzen C.J., Holmes R.W. and Strickland J.D.H. 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer. 30: 3-15 https://doi.org/10.1093/icesjms/30.1.3
  2. 이춘환. 1992. 엽록소에서 방출되는 형광의 분석을 통한 광합성 연구. 제4권 생화학 총설집. pp.265-271
  3. 정영호. 1968. 한국동식물도감 제9권. 식물편(담수조류). 문교부. 삼화출판사
  4. 정준. 1993. 한국담수조류도감. 아카데미서적
  5. Ajlani G., Vernotte C., Dimagno L. and Haselkorn R. 1995. Phycobilisome core mutants of Synechosystis PCC 6803. Biochim, Biophys. Acta 1231: 189-196 https://doi.org/10.1016/0005-2728(95)00086-X
  6. Bentley-Mowat J.A. and Reid S.M. 1977. Survival of marine phytoplankton in high concentration of heavy metals and uptake of copper. J. Exp. Mar. Biol. Ecol. 26: 249-264 https://doi.org/10.1016/0022-0981(77)90085-5
  7. Bringman G. and Kuhn R. 1978. Survey of algal species sensitivity to a wide range of toxicants. Mitt. Int. Verein. Limnol. 21: 275-284
  8. Bruce D., Brimble S. and Bryant D.A. 1989. State transition in a phycobilisome-less mutant of the cyanobacterium Synechococcus sp. PCC 7002. Biochim. Biophys. Acta 974: 66-73 https://doi.org/10.1016/S0005-2728(89)80166-5
  9. Bryant D.A. 1994. The molecular biology of cyanobacteria. Kluwer Academic press, Dordrecht
  10. Carmichael W.W. 1993. Disease related to freshwater blue-green algae toxins and control measures. In: Falconer I.R. (ed.), Algal toxins in seafood and drinking water. Cambridge Academic Press, Cambridge. pp. 187-209
  11. Codd G.A. 2000. Cyanobacterial toxins, the perception of water quality and the prioritisation of eutrophication control. Ecol. Engineer. 16: 51-60
  12. Eullaffroy P. and Vernet G. 2003. The F684/F735 chlorophyll fluorescence ratio; a potential tool for rapid detection and determination of herbicide phytotoxicity in algae. Water Res. 37: 1983-1990 https://doi.org/10.1016/S0043-1354(02)00621-8
  13. Jeffrey S.W. 1997. Application of pigment methods to oceanography. In: Jeffrey S.W., Mantoura R.F.C. and Wright S.W. (eds), Phytoplankton pigments in oceanography. UNESCO Publ. pp. 127-166
  14. Jeffrey S.W. and Humphrey C.F. 1975. New spectrometric equations for determining chlorophyll a, b, and c, in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanzen. 167: 191-194 https://doi.org/10.1016/S0015-3796(17)30778-3
  15. Jeffrey S.W. and LeRoi J.-M. 1997. Simple procedure for growing SCOR reference microalgal cultures. In: Jeffrey S.W., Mantoura R.F.C. and Wright S.W. (eds), Phytoplankton pigments in oceanography. UNESCO Publ. pp. 181-205
  16. Juneau P., Dewez D., Matsui S., Kim S.G. and Popovic R. 2001. Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry. Chemosphere 45: 589-598 https://doi.org/10.1016/S0045-6535(01)00034-0
  17. Lichenthaler H.H. 1988. Application of chlorophyll fluorescence. Kluwer Academic Press, Dordrecht
  18. Margalef R. 1983. Ecologia del fitoplancon in Limnologia. Omega, Barcelona. pp. 247-330
  19. Mizuno T. 1977. Illustrations of the freshwater plankton of Japan. Hoikusha Publ
  20. Murakami A. 1997. Quantitative analysis of 77K fluorescence emission spectra in Synechocystis sp. PCC 6714 and Chlamydomanas reinhardtii with variable PS I / PS II stoichiometries. Photosynth. Res. 53: 141-148 https://doi.org/10.1023/A:1005818317797
  21. Murata N. and Satoh K. 1986. Absorption and fluorescence emission by intact cells, chloroplasts and chlorophyll-protein complexes. In: Gonindjee, Amesz J. and Fork D.C. (eds), Light emission by plants and bacteria. New York Academic Press, New York. pp. 137-159
  22. Namikoshi M. and Rinehart K.L. 1996. Bioactive compounds produced by cyanobacteria. J. Indus. Microbial. 17: 373-384 https://doi.org/10.1007/BF01574768
  23. Seely G.R. and Connolly J.S. 1986. Fluorescence of photosynthetic pigments in vitro. In: Conindjee, Amesz J. and Fork D.C. (eds), Light emission by plants and bacteria. New York Academic Press, New York. pp. 99-133
  24. Song E,K., Zulfugarov I.S., Kim J.-H., Kim E.H. Lee W.S. and Lee C.-H. 2004. Selection and characterization of transposon tagging mutants of Synechocystis sp. PCC 6803 sensitive to high-light and oxidative stresses. J. Plant Biol. 47: 289-299 https://doi.org/10.1007/BF03030543
  25. Stauber J.L. and Florence T.M. 1987. Mechanism of toxicity of ionic copper and copper complexes to algae. Mar. Biol. 94: 511-519 https://doi.org/10.1007/BF00431397
  26. Velzeboer R., Drikas M., Donati C, Burch M. and Steffensen D. 1995. Release of geosmin Anabaena circinalis following treatment with aluminium sulphate. Water Sci. Tech. 31: 187-194
  27. Yentch C.S. and Phinney D.A. 1985. Spectral fluorescence: a taxonomic tool for studying the structure of phytoplankton populations. J. Plankton Res. 7: 617-632 https://doi.org/10.1093/plankt/7.5.617
  28. Misra H.S. and Mahajan S.K. 2000. Excitation energy transfer from phycobilisomes to photosystems: a phenomenon associated with the temporal separation of photosynthesis and nitrogen fixation in a cyanobacterium Plecionema boryamum. Biochim. Biophys. Acta 1459: 139-147 https://doi.org/10.1016/S0005-2728(00)00123-7

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