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Molecular Sexing Using SRY and ZF Genes in Pigs

돼지 SRY와 ZF 유전자를 이용한 성판별 기법

  • Cho, I.C. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Kang, S.Y. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Lee, S.S. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Choi, Y.L. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Ko, M.S. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Oh, M.Y. (Department of Life Science, College of Natural Sciencs, Cheju Natioanl University) ;
  • Han, Sang-Hyun (National Institute of Subtropical Agriculture, R.D.A.)
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 강승률 (농촌진흥청 난지농업연구소) ;
  • 이성수 (농촌진흥청 난지농업연구소) ;
  • 최유림 (농촌진흥청 난지농업연구소) ;
  • 고문석 (농촌진흥청 난지농업연구소) ;
  • 오문유 (제주대학교 생명과학과) ;
  • 한상현 (농촌진흥청 난지농업연구소)
  • Published : 2005.06.30

Abstract

A method for sex determination of pigs was examined using polymerase chain reaction(PCR). Sex determining region Y(SRY) gene encoded on Y chromosome plays a key role for primary male development. Zinc finger X-Y(ZFX-ZFY) gene, one of the X-V homology gene group was found on the X and Y chromosomes, respectively, We tested for molecular sexing by amplification patterns of SRY and ZF genes. Genomic DNAs from various resources including porcine hairs and semen collected from domestic pig breeds and native pigs was used for PCR assay of each gene. The amplified products for porcine SRY gene were yielded only in males but not in females. On the other hand, two differential patterns were observed in amplification of ZF gene reflecting the chromosomal dimorphism by a length polymorphism between X and Y chromosomes. Of both, a common band was detected in all individuals tested so that this band might be amplified from ZFX gene as a PCR template, but another is specific for males indicated that from ZFY. The result of PCR assay provides identical information to that from investigation of phenotypic genders of the pigs tested. We suggest that this PCR strategy to determine porcine sexes using comparison of the amplification patterns of the SRY gene specific for Y chromosome and the dimorphic ZF gene between X and Y chromosomes may be a rapid and precise method for discrimination of two sexes and applied to DNA analysis of small samples such as embryonic blastomere, semen, and hairs.

Keywords

Chromosome;Pig;Polymerase chain reaction;Sex determination;SRY;ZF

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