Expression of Bcl-2 Family in 4-Nitroquinoline 1-Oxide-Induced Tongue Carcinogenesis of the Rat

백서 혀에서의 4-nitroquinoline 1-oxide 유도 발암과정에서 Bcl-2 계 유전자의 발현

  • Choi, Jae-Wook (Dept. of Oral Medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Sung-Su (Dept. of Anesthesiology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Lee, Geum-Sug (Dept. of Oral Medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Byung-Gook (Dept. of Oral Medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Jae-Hyeong (Dept. of Oral Medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Kook, Eun-Byul (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Jang, Mi-Sun (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Ko, Mi-Kyeong (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Jung, Kwon (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Choi, Hong-Ran (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Ok-Joon (Dept. of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University)
  • 최재욱 (전남대학교 치의학전문대학원 구강내과학교실, 전남대학교 치의학연구소) ;
  • 정성수 (전남대학교 치의학전문대학원 마취통증의학교실, 전남대학교 치의학연구소) ;
  • 이금숙 (전남대학교 치의학전문대학원 구강내과학교실, 전남대학교 치의학연구소) ;
  • 김병국 (전남대학교 치의학전문대학원 구강내과학교실, 전남대학교 치의학연구소) ;
  • 김재형 (전남대학교 치의학전문대학원 구강내과학교실, 전남대학교 치의학연구소) ;
  • 국은별 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소) ;
  • 장미선 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소) ;
  • 고미경 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소) ;
  • 정권 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소) ;
  • 최홍란 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소) ;
  • 김옥준 (전남대학교 치의학전문대학원 구강병리학교실, 전남대학교 치의학연구소)
  • Published : 2005.09.30

Abstract

The number of patients with tongue carcinoma is increasing rapidly among young individuals in many parts of the world. Oral carcinoma progresses from hyperplastic lesion through dysplasia to invasive carcinoma and the concept of "field cancerization" with molecular alteration has been suggested for oral cavity carcinogenesis. Significant improvement in treatment and prognosis will depend on more detailed understanding of the multi-step process leading to cancer development. To induce tongue carcinoma in rat by 4-NQO, each drinking water was made to 10 ppm, 25 ppm, 50 ppm and control (only D.W. without 4-NQO). Specimens were classified into 4 groups such as control, I (mild & moderate dysplasia), II (severe dysplasia and carcinoma in situ), III (carcinoma). The mRNA expressions of Bcl-2 family were evaluated by RT-PCR technique. For anti-apoptotic Bcl-2 family, mRNA expression of Bcl-w was down-regulated in all stages of tongue carcinogenesis model. However, mRNA expression of Bcl-2 was up-regulated. For pro-apoptotic Bcl-2 family, all members were down-regulated in all stages of tongue carcinogenesis model except for Bad mRNA in group III. In terms of BH3 only protein, mRNA expressions of Bok and Mcl-1 were down regulated in all stages of specimen, but Bmf in group II and BBC3 in group III were up-regulated. Our current findings demonstrated the involvements of mRNA expression of Bcl-2 family in multi-step tongue carcinogensis. This highlights the necessity for continued efforts to discover suitable biomakers (Bcl-2 family) for early diagnosis of the disease, and to understand its pathogenesis as a first step in improving methods of treatment. The discovery of these potential biomarkers and molecular targets for cancer diagnostics and therapeutics has the potential to significantly change the clinical approach and outcome of the disease.

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