Cultural Conditions for Pretense Production by a feather-Degrading Bacterium, Bacillus megaterium F7-1

우모분해세균 Bacillus megaterium F7-1에 의한 단백질 분해효소 생산에 영향을 미치는 배양조건

  • Son Hong-Joo (Department of Biotechnology, Miryang National University)
  • 손홍주 (밀양대학교 생명공학과)
  • Published : 2005.12.01

Abstract

The effects of inorganic salts and feather concentrations on pretense production by Bacillus megaterium F7-1 were investigated. Pretense production was dependent on the presence of phosphates in the medium. Supplementation of medium with calcium ion slightly increased protease production. The highest protease production was obtained at $1.4\%$ feather. The optimal medium contained $2.0\%$ glucose, $0.8\%$ skim milk, $0.06\%\;K_{2}HPO_{4}\%,\;0.04\%\;KH_{2}PO{4},\;0.06\%\;NaCl,\;0.03\%\;MgCl_{2}\cdot6H_{2}O,\;0.002\%\;CaCl_{2}\cdot2H_{2}O,\;and\;1.4\%$ whole feather. By using this optimized medium, increased production of the protease was achieved compared with the cases of using basal medium.

Keywords

Bacillus megaterium;feather;protease

References

  1. Cheng, S. W., H. M. Hu, S. H. Shen, H. Takai, M. Asano, and Y. C. Tsai. 1995. Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-J. Biosci. Biochem. Biotechnol. 59: 2239-2243 https://doi.org/10.1271/bbb.59.2239
  2. Kim, Y. B., J. B. Lee, K. S. Sung, and N. H. Lee. 1998. Effects of physical processing on protein content and pepsindigestibility offeather meals. Kor. J. Anim. Sci. 40: 103-110
  3. Ramnani, P. and R. Gupta. 2004. Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RGI using statistical methods involving response surface methodology. Biotechnol. Appl. Biochem. 40: 191-196 https://doi.org/10.1042/BA20030228
  4. Onifade, A. A., N. A. AI-Sane, A. A. AI-Musallam, and S. AI-Zarban. 1998. A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresource Technol. 66: 1-11 https://doi.org/10.1016/S0960-8524(98)00033-9
  5. Lahl, W. J. and S. D. Braun. 1994. Enzymatic production of protein hydrolysates for food use. Food Technol. October 68-71
  6. Charney, J. and R. M. Tomarelli. 1947. A colourimetric method for the determination of proteolytic activity of duodenal juice. J. Biol. Chem. 171: 501-505
  7. Son, H. J., Y. G Kim, and Y. K. Park. 2004. Isolation and identification of feather-degrading bacteria for biotechnological applications of keratinaceous protein waste. J. Life Sci. 14: 229-234 https://doi.org/10.5352/JLS.2004.14.2.229
  8. EI-Refai, H. A., M. A. AbdelNaby, A. Gaballa, M. H. EIAraby, and A. F. Fattah. 2005. Improvement of the newly isolated Bacillus pumilis FH9 keratinolytic activity. Process Biochem. 40: 2325-2332 https://doi.org/10.1016/j.procbio.2004.09.006
  9. Lin, X., C. C. Lee, E. S. Casale, and J. C. H. Shih. 1992. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environ. Microbiol. 58: 3271-3275
  10. Papadopoulos, M. C., A. R. EI Boushy, and A. E. Roodbeen. 1985. The effect of varying autoclaving conditions and added sodium hydroxide on amino acid content and nitrogen characteristics of feather meal. J. Sci. Food Agric. Abstr. 36: 1219-1226 https://doi.org/10.1002/jsfa.2740361204
  11. Son, H. J. G T. Park, and Y. G Kim. 2004. Production of a keratinolytic protease by a feather-degrading bacterium, Bacillus megaterium F7-1. Kor. J. Microbiol. 40: 43-48
  12. Singh, C. J. 2002. Optimization of an extracellular protease of Chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes. Mycophathologia 156: 151-156
  13. Korea Chicken Council. 2002. www.chicken.or.kr/3data/do.htm
  14. Oyeka, C. A. and H. C. Gugnani. 1997. Keratin degradation by Scytalidium species and Fusarium solani. Mycoses 41: 73-76
  15. Rao, M. B., A. M. Tanksale, M. S. Ghatge, and V. V. Deshpande. 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 62: 597-635
  16. Papadopoulos, M. C. 1985. Processed chicken feathers as feedstuff for poultry and swine. A review. Agric. Wastes 14: 275-290 https://doi.org/10.1016/S0141-4607(85)80009-3