Assessment of Inactivation for Campylobacter spp. Attached on Chicken Meat

계육에 오염된 Campylobacter 균의 불활성화 평가

  • Jang Keum-Il (Department of Food Science and Technology Chung-buk National University) ;
  • Jeong Heon-Sang (Department of Food Science and Technology Chung-buk National University) ;
  • Kim Chung-Ho (Department of Food and Nutrition, Seowon University) ;
  • Kim Kwang-Yup (Department of Food Science and Technology Chung-buk National University)
  • Published : 2005.12.01

Abstract

The inactivation efficiency of Campylobacter jejuni were assessed in vitro and in vivo using confocal laser microscopy and flow cytometry. C. jejuni cells were inactivated with $1\%$ (w/v) trisodium phosphate (TSP) and the live cells and inactivated cells were distinguished by staining with LIVE/DEAD BacLight Bacteria Viability fluorescent probe. After treatment of TSP for 5 min, most of C. jejuni cells turned to coccoid form from original spiral shape. C. jejuni cells lost total cell viability in the absence of organic nutrients but did not lost total cell viability in the presence of organic nutrients. In vivo test, C. jejuni cells turned to viable but non-culturable (VBNC) form after TSP treatment and remained alive on chicken skin. C. jejuni cells attached on chicken meat would transform to coccoid form by sanitizer treatment, but could possibly be alive by the benefits of organic nutrients present in chicken meat.

Keywords

Campylobacter jejuni;confocal microscopy;flow cytometry;sanitizer;trisodium phosphate

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