Interaction between IgE-Dependent Histamine-Releasing Factor and Triosephosphate Isomerase in HeLa Cells

HeLa 세포에서 IgE-dependent Histamine-Releasing Factor와 Triosephosphate Isomerase의 상호작용 규명

  • Moon Ji-Ae (College of Pharmacy, Ewha Woman's University) ;
  • Kim Hwa-Jung (College of Pharmacy, Ewha Woman's University) ;
  • Lee Kyunglim (College of Pharmacy, Ewha Woman's University)
  • 문지애 (이화여자대학교 약학대학) ;
  • 김화정 (이화여자대학교 약학대학) ;
  • 이경림 (이화여자대학교 약학대학)
  • Published : 2005.12.01

Abstract

IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K-ATPase. To understand the molecular mechanism of the regulation of Na, K-ATPase activity by HRF, we investigated the interaction between HRF and TPI since TPI was obtained as HRF-interacting protein in HeLa cDNA library, using yeast two hybrid screening. Domain mapping study of the interaction between HRF and TPI revealed that the C-terminal region of the residue 156-249 of TPI is involved in the interaction with HRF. The interaction between HRF and TPI was confirmed by immunoprecipitation from HeLa cell extracts. Our results suggest that TPI is a HRF-binding protein and the interaction between HRF and TPI nay thus affect Na, K-ATPase activity.

Keywords

HRF;TPI;yeast two hybrid;Na;K-ATPase

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