Effects of Abiotic Stresses on Cell Cycle Progression in Tobacco BY-2 Cells

  • Jang, Su Jin ;
  • Shin, Sung Hae ;
  • Yee, Sung Tae ;
  • Hwang, Baek ;
  • Im, Kyung Hoan ;
  • Park, Ky Young
  • Received : 2005.04.08
  • Accepted : 2005.05.18
  • Published : 2005.08.31

Abstract

Mild stresses such as high temperature ($30^{\circ}C$) or a low $H_2O_2$ concentration induced transient cell cycle arrest at G1/S or G2/M depending on the cell cycle stage at which the stress was applied. When stresses were introduced during G0 or G1, the G1/S checkpoint was mainly used; when stresses were introduced after S phase, G2/M was the primary checkpoint. The slowing of cell cycle progression was associated with transient delays in expression of A-, B-, and D-type cyclins. The delay in expression of NtcycA13, one of the A-type cyclins, was most pronounced. The levels of expression of Ntcyc29 (a cyclin B gene) and of CycD3-1 differed most depending on the applied stress, suggesting that different cellular adjustments to mild heat and a low concentration of $H_2O_2$ are reflected in the expression of these two cyclins.

Keywords

Cell Cycle;Cyclins;Heat Stress;Hydrogen Peroxide;Tobacco BY-2 Cells

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Acknowledgement

Supported by : Korea Science and Engineering Foundation