Gamma-Irradiation and Doxorubicin Treatment of Normal Human Cells Cause Cell Cycle Arrest Via Different Pathways

  • Lee, Seong Min (School of Life Sciences and Biotechnology, Korea University) ;
  • Youn, BuHyun (School of Molecular Biosciences, Washington State University) ;
  • Kim, Cha Soon (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Chong Soon (Radiation and Health Research Institute) ;
  • Kang, ChulHee (School of Molecular Biosciences, Washington State University) ;
  • Kim, Joon (School of Life Sciences and Biotechnology, Korea University)
  • Received : 2005.04.12
  • Accepted : 2005.08.18
  • Published : 2005.12.31

Abstract

Ionizing radiation and doxorubicin both produce oxidative damage and double-strand breaks in DNA. Double-strand breaks and oxidative damage are highly toxic and cause cell cycle arrest, provoking DNA repair and apoptosis in cancer cell lines. To investigate the response of normal human cells to agents causing oxidative damage, we monitored alterations in gene expression in F65 normal human fibroblasts. Treatment with ${\gamma}$-irradiation and doxorubicin altered the expression of 23 and 68 known genes, respectively, with no genes in common. Both agents altered the expression of genes involved in cell cycle arrest, and arrested the treated cells in $G_2M$ phase 12 h after treatment. 24 h after ${\gamma}$-irradiation, the percentage of $G_1$ cells increased, whereas after doxorubicin treatment the percentage of $G_2M$ cells remained constant for 24 h. Our results suggest that F65 cells respond differently to ${\gamma}$-irradiation- and doxorubicin-induced DNA damage, probably using entirely different biochemical pathways.

Keywords

${\gamma}$-Irradiation;Differential Gene Expression;Doxorubicin;Normal Human Fibroblasts

Acknowledgement

Supported by : KRF

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