Characterization of Rice Mutants with Enhanced Susceptibility to Rice Blast

  • Kim, Hye-Kyung (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Sang-Kyu (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Cho, Jung-Il (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Sichul (Pohang University of Science and Technology) ;
  • An, Gynheung (Pohang University of Science and Technology) ;
  • Jwa, Nam-Soo (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Kim, Byung-Ryun (National Institute of Crop Sciences, Rural Development Administration) ;
  • Cho, Young-Chan (National Institute of Crop Sciences, Rural Development Administration) ;
  • Han, Seong-Sook (National Institute of Crop Sciences, Rural Development Administration) ;
  • Bhoo, Seong-Hee (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Youn-Hyung (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Hong, Yeon-Kyu (Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Yi, Gihwan (Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Dae-Sup (Turf & Environment Research Institute, Samsung Everland Inc.) ;
  • Hahn, Tae-Ryong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Jeon, Jong-Seong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2005.07.14
  • Accepted : 2005.08.29
  • Published : 2005.12.31

Abstract

As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the $F_2$ segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the $F_2$ population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 $F_2$ plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor.

Keywords

Blast Resistance;Hwayeong;NB-LRR;Rice;Pib Mutation

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)

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