Crystallization and Preliminary X-Ray Crystallographic Analysis of PAS Factor from Vibrio vulnificus

  • Lee, Jun Hyuck (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kim, Soo Young (Department of Microbiology, Chonnam National University Medical School) ;
  • Rho, Seong-Hwan (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Im, Young Jun (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kim, Young Ran (Department of Microbiology, Chonnam National University Medical School) ;
  • Kim, Mun-Kyoung (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kang, Gil Bu (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Rhee, Joon Haeng (Department of Microbiology, Chonnam National University Medical School) ;
  • Eom, Soo Hyun (Department of Life Science, Gwangju Institute of Science and Technology)
  • Received : 2005.06.27
  • Accepted : 2005.08.17
  • Published : 2005.12.31

Abstract

Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a = 56.1, b = 74.4, $c=80.0{\AA}$, ${\alpha}={\beta}={\gamma}=90^{\circ}$. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was $1.9{\AA}$ using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.

Keywords

PAS Factor;Secretion;Vibrio vulnificus;X-Ray Crystallography

Acknowledgement

Supported by : Ministry of Health and Welfare

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