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cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle

  • Kim, Chul Wook (Department of International Livestock Industry, Jinju National University) ;
  • Chang, Kyu Tae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hong, Yeon Hee (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Jung, Won Yong (Department of International Livestock Industry, Jinju National University) ;
  • Kwon, Eun Jung (Department of International Livestock Industry, Jinju National University) ;
  • Cho, Kwang Keun (Department of International Livestock Industry, Jinju National University) ;
  • Chung, Ki Hwa (Department of International Livestock Industry, Jinju National University) ;
  • Kim, Byeong Woo (Division of Applied Life Science Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Jung Gyu (Division of Applied Life Science Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Yeo, Jung Sou (Institute of Biotechnology Yeungnam University) ;
  • Kang, Yang Su (Gyeongsangnam-do Agricultural Research and Extension Servies) ;
  • Joo, Young Kuk (Institute of Gyeongnam Province Advanced Swine Research)
  • Received : 2004.11.04
  • Accepted : 2005.03.04
  • Published : 2005.08.01

Abstract

By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies. It was suggested that there are many isoforms of these genes in the genome of swine. Also, results of northern blotting to investigate whether these genes were expressed in grown muscle, using GF I, III, and V indicated that all the genes were much more expressed in the muscle of swine with body weight of 90 kg. Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. These genes were not expressed in the propagation and fat tissues, but were expressed in the muscle tissue. To determine the mechanism of muscle growth, further studies should be preceded using the 3 specific genes related to muscle growth, that is GF I, III, and V.

Keywords

Muscle Genes;Swine;Expression Profiles;cDNA Microarray

Acknowledgement

Supported by : Rural Development Administration

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