Estimation of the Cumulative Power of Discrimination in Haimen Chicken Populations with Ten Microsatellite Markers

  • Olowofeso, O. ;
  • Wang, J.Y. ;
  • Shen, J.C. ;
  • Chen, K.W. ;
  • Sheng, H.W. ;
  • Zhang, P. ;
  • Wu, R.
  • Received : 2004.09.26
  • Accepted : 2005.01.28
  • Published : 2005.08.01


To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00${\pm}$0.33 (Rugao) to 4.90${\pm}$0.48 (Cshiqishi) and across populations for all loci was 4.60${\pm}$0.20, while (H) ranged from 0.65${\pm}$0.03 (Rugao) to 0.69${\pm}$0.03 (Jiangchun) among loci and across populations, (H) was 0.67${\pm}$0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.


Estimation;Cumulative Power of Discrimination;Haimen Chickens;Markers


  1. Bartfai, R., S. Egedi, G. H. Yue, B. Kovacs, B. Urbanyi, G. Tamas, L. Horvath and L. Orban. 2003. Genetic analysis of two common carp broodstocks by random amplified polymorphic DNA (RAPD) and microsatellite markers. J. Aquaculture 219:157-167.
  2. Botstein, D., R. L. White and M. Skolnick. 1980. Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331.
  3. Chen, G. H., X. S. Wu, D. Q. Wang, J. Qin, S. L. Wu, Q. L. Zhou, F. Xie, R. Cheng, Q. Xu, B. Liu, X. Y. Zhang and O. Olowofeso. 2004. Cluster analysis of 12 Chinese native chicken populations using microsatellite markers. Asian-Aust. J. Anim. Sci. 17(8):1047-1052.
  4. Chen, H., L. Leibenguth and Z. Bi-cai. 2002. DNA fingerprinting in five animal species using microsatellite probes. In: Proceedings of the 8th National Symposium on Animal Genetic Markers. 8(1):67-71, Oct., Yangling, China.
  5. Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.
  6. Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular cloning- A laboratory manual. Vol. 2, 2nd edition. Cold Spring Harbour, Laboratory Press, USA.
  7. Sano, A., Z. Huiling, M. Kimura, C. Hong and K. Nosawa. 1997. Genetic variability in commercial Quail populations in Shaanxi, China. In: Studies on Animal Genetic Resources in China, Edited by Chang Hong, Nov., Pages 223-229.
  8. Wimmers, K., S. Ponsuksili, T. Hardge, A. Valle-Zarate, P. K. Mathur and P. Horst. 2000. Genetic distinctness of African, Asian and South American local chickens. Anim. Genet. 31:159-165.
  9. Wu, X. S. 2004. Study on genetic diversity in Chinese indigenous chicken breeds using microsatellite markers and their relationships between performance and microsatellite loci. Ph.D. Thesis, Yangzhou University, Yangzhou, China.
  10. Chenyambuga, S. W., O. Hanotte, J. Hirbo, P. C. Watts, S. J. Kemp, G. C. Kifaro, P. S. Gwakisa, P. H. Petersen and J. E. O. Rege. 2004. Genetic characterization of indigenous Goats of Subsaharan Africa using microsatellite DNA markers. Asian-Aust. J. Anim. Sci. 17(4):445-452.
  11. Emara, M. G. and H. Kim. 2003. Genetic markers and their application in Poultry Breeding. Poult. Sci. 82:952-957.
  12. Ponsuksili, S., K. Wimmers, F. Scmoll, P. Horst and K. Schellander. 1999. Comparison of multilocus DNA fingerprints and microsatellites in an estimate of genetic distances in chicken. J. Hered. 6:656-659.
  13. Barker, J. S. F. 1994. A global protocol for determining genetic distances among domestic livestock breeds. In: Proceedings of the 5th World Congress of Genetics Applied to Livestock Production. 21:501-508.
  14. Miller, S. A., D. D. Dykes and H. F. Plosky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215.
  15. Shen, J. C. 2004. Study on genetic diversity of nine indigenous Chinese chicken breeds using microsatellite markers. M.Sc. Thesis, Yangzhou University, Yangzhou, China.
  16. Vanhala, T., M. Tuiskala-Haavisto, K. Elo, J. Vilkki and A. Maki-Tanila. 1998. Evaluation of genetic variability and genetic distances between eight chicken lines using microsatellite markers. Poult. Sci. 77:783-790.
  17. Fan, B., B. Liu and K. Li. 2002. The application of microsatellite DNA marker in animal individual verification and breed assignment. In: Proceedings of the 8th National Symposium on Animal Genetic Markers. 8(1):35-38, Oct., Yangling, China.
  18. Yang, Z. P., H. Chang, W. Sun, R. Q. Gen, Y. J. Mao and K. Tsunoda. 2004. A comparison of two kinds of markers applied in analysis of genetic diversity in Sheep and Goat populations. Asian-Aust. J. Anim. Sci. 17(7):892-896.
  19. Zhang, X., F. C. Leung, D. K. O. Chan, G. Yang and C. Wu. 2002. Genetic diversity of Chinese native chicken breeds based on protein polymorphism, random amplified polymorphic DNA and microsatellite polymorphism. Poult. Sci. 81:1463-1472.

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