Characteristics of Extraction of Daidzein and Genistein in Soybean Using Sub/Supercritical Fluids

아임계/초임계 유체를 이용한 콩에 포함된 Daidzein과 Genistein의 추출특성

  • Choi, Du Young (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Zheng, Jinzhu (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
  • 최두영 (초정밀생물분리기술연구센터, 인하대학교 화학공학과) ;
  • 정금주 (초정밀생물분리기술연구센터, 인하대학교 화학공학과) ;
  • 노경호 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
  • Received : 2005.01.13
  • Accepted : 2005.07.11
  • Published : 2005.10.10

Abstract

Daidzein and genistein were extracted from Korean soybean by supercritical $CO_2$ and sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The retention time, retention factor, column efficiency, column selectivity and resolution of aglycons were compared with the change in the temperature and pressure of supercritical fluid and ethanol concentration. The characteristics of extraction of daidzein and genistein were more affected by ethanol concentration using supercritical $CO_2$. The most desirable extraction yield was obtained by supercritical $H_2O$ with $400^{\circ}C$ and 250 bar. Generally, the extraction yield of aglycons increased over 10 times using supercritical $CO_2$ than sub/supercritical $H_2O$.

Acknowledgement

Supported by : 인하대학교, 한국과학기술연구원

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