Extraction of Whitening Agents from Natural Plants and Whitening Effect

천연물에 포함된 미백성분의 추출 및 미백효과

  • Jin, Yinzhe (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Ahn, So Young (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Hong, Eun Suk (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Li, Guang hua (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Kim, Eun-Ki (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Row, kyung Ho (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University)
  • 김은철 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 안소영 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 홍은숙 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 이광화 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 김은기 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 노경호 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부)
  • Received : 2004.10.19
  • Accepted : 2005.02.22
  • Published : 2005.06.10

Abstract

The extracts from natural and fermented products such as Artemisia plants, Rhodiola Salientness, fermented soybeans and soybean paste were used to investigate the whitening effect. 10 g of Artemisia plant were added to 300 mL of ethanol and extracted by sonification at room temperature for 3 h. The extract was further partitioned by the equal volume percent in the order of the n-hexane, chloroform and ethyl acetate. 5 g of Rhodiola salientness was also added to 150 mL of methanol and extracted at the room temperature for 12 h. The effluents from a chromatographic column ($3.9{\times}250mm$, $C_{18}$, $15{\mu}m$) were collected and concentrated in two parts. The extraction of fermented soybeans and soybean paste were done by 60% ethanol. In this work, tyrosinase inhibitory activity and melanin inhibitory effect were measured to confirm the whitening effect. The water layer of Artemisia princeps Pampan showed the good inhibitory of antioxidant, while the hexane layer of Artemisia iwayomogi Kitamura and the chloroform layer of Artemisia princeps Pampan had the excellent melanin inhibitory effect. The Rhodiola salientness had the superior whitening effect to the arbutin in in-vivo melanin production ratio assay. However, the fermented soybeans and soybean paste did not show any whitening effect.

Acknowledgement

Supported by : 인하대학교

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