Determination of tetracycline antibiotics in food

식품 중 테트라싸이클린계 항생물질의 분석

  • Park, Dongmi (Residue & Chemicals Division, Korea Food & Drug Administration) ;
  • Jeong, Jiyoon (Residue & Chemicals Division, Korea Food & Drug Administration) ;
  • Chang, Moonik (Residue & Chemicals Division, Korea Food & Drug Administration) ;
  • Im, Moohyeog (Residue & Chemicals Division, Korea Food & Drug Administration) ;
  • Park, Kunsang (Residue & Chemicals Division, Korea Food & Drug Administration) ;
  • Hong, Mooki (Residue & Chemicals Division, Korea Food & Drug Administration)
  • 최동미 (식품의약품안전청, 잔류화학물질과) ;
  • 정지윤 (식품의약품안전청, 잔류화학물질과) ;
  • 장문익 (식품의약품안전청, 잔류화학물질과) ;
  • 임무혁 (식품의약품안전청, 잔류화학물질과) ;
  • 박건상 (식품의약품안전청, 잔류화학물질과) ;
  • 홍무기 (식품의약품안전청, 잔류화학물질과)
  • Received : 2005.04.08
  • Accepted : 2005.04.21
  • Published : 2005.06.25


A selective method of high performance liquid chromatography with UV detector has been applied to determine 4 tetracycline antibiotics in the animal food, simultaneously. The targets were chlortetracycline (CTC), doxycycline (DC), oxytetracycline (OTC), and tetracycline (TC) that are used routinely in veterinary medicine for prevention and control of disease. Food samples were beef, pork, chicken, milk, whole egg, flatfish (Limanda yokohamae), jacopever (Sebastes hubbsi), seabream (Chrysophrys major), eel (Anguilla japonica) and lobster (Hommarus americanus). After extracting food samples with 20% trichloroacetic acid and McIlvaine buffer, they were purified by a $C_18$ SPE cartridge with 0.01M methanolic oxalic acid solution. The concentrated residue was re-dissolved in methanol, filtered, cleaned up and analyzed on a $C_18$ column. The mobile phase was a mixture of 0.01M oxalic acid and acetonitrile with a gradient ratio from 85:15 to 60:40. The UV wavelength was 365 nm. The overall recoveries were ranged from 71% to 98% and the limit of detections were 0.022 for CTC, 0.012 for DC and OTC and 0.009 mg/kg for TC at signal/noise > 3, respectively. As results, CTC, DC and TC were not detected in all selected food samples, however, OTC was detected in meat and fishes. The determined level of OTC was 0.04 ppm for pork, 0.17 ppm for flatfish and 0.05 and 0.08 ppm for jacopever, that were within the Maximum Residue Limits (MRLs) in the food.


  1. 동물용의약품등편람, 한국동물약품협회, 2001
  2. The Merck Veterinary Manual (
  3. 이장락, '동물약품해설', 서울대학교출판부, 1991
  4. 신형태, 한국영양학회, 7, 53-70(1983)
  5. 배합사료제조용 동물용 의약품 등 사용기준, 국립수의과학검역원 고시 제2002-6호, 2002.09.16
  6. 동물용 의약품 안전사용기준, 국립수의과학검역원 고시 제2003-4호, 2002.07.26
  7. 양식어업인을 위한 수산용약품사용안내, 국립수산과학원, 1996
  8. 식품공전, 식품의약품안전청, 2002
  9. 식품중 동물용의약품 잔류허용기준(행정간행물등록번호: 11-1470000-000532-01), 식품의약품안전청 잔류화학물질과, 2004
  10. 이선녀, 홍종기, 분석과학, 17, 43A-58A(2004)
  11. H. Oka, Y. Ito and H. Matsumoto, J. Chromatogr. A, 882, 109-133(2002)
  12. S. Yang and K. Carson, Water, Research, 38, 3155-3166(2004)
  13. A. Cinquina, F. Long, G. Anastasi and L. Cozzani, J. Chromatogr. A, 987, 227-233(2003)
  14. M. Hernandez, F. Borrull and M. Calull, Trends in Anal. Chem., 22, 416-427(2003)
  15. FAO/IAEA 동물용의약품잔류분석기술 훈련자료집, 국립수의과학검역원, 2004
  16. W. Moats, K. Anderson, J. Rushing and D. Wesen, Am. J. Vet. Res., 56(6), 795-800(1995)
  17. J. MacNeil, V. Martz, G. Korsrud, C. Salisbury, H. Oka, R. Epstein and C. Barnes, J. AOAC International, 79, 405-417(1996)
  18. M. Cherlet, M. Schelkens, S. Croubels and P. Backer, Anal. Chim. Acta., 492, 199-213(2003)
  19. 정석찬, '축산용 항생제 관리시스템 구축', 국립수의과학검역원, 식품의약품안전청, 2003
  20. 유해사료의 범위와 기준중개정, 농림부 고시 제2004-72호, 2004.12.10
  21. 배합사료제조용 동물용 의약품 등 사용기준 고시개정(안) 입안예고, 국립수의과학검역원 공고 제2005-116호, 2005.02.03