Effect of Dietary Addition of Turkish Propolis on the Growth Performance, Carcass Characteristics and Serum Variables of Quail (Coturnix coturnix japonica)

  • Denli, Muzaffer (Department of Animal Science, Cukurova University) ;
  • Cankaya, S. (Department of Animal Science, Cukurova University) ;
  • Silici, S. (Department of Animal Health, Erciyes University) ;
  • Okan, F. (Department of Animal Science, Cukurova University) ;
  • Uluocak, A.N. (Department of Animal Science, Cukurova University)
  • 투고 : 2004.09.26
  • 심사 : 2005.01.15
  • 발행 : 2005.06.01


We examined the effect of dietary Turkish propolis and flavomycin on growth performance, carcass characteristics, internal organ weights and some serum variables in quail (Coturnix coturnix japonica) birds. One hundred and fifty day-old quails were randomly divided into five groups, with ten replicate pens per treatment and three birds per pen. One group received the basal diet (antibiotic-free), the control. The flavomycin at 10 mg/kg diet and propolis at 0.5, 1 and 1.5 g/kg diet were added to the basal diet. Body weight gain, feed consumption and feed efficiency were determinated weekly. Carcass characteristics, internal organ weights and serum variables were determinated at the end of the study (35 day). The results showed that body weight gain, feed efficiency and carcass weight were improved significantly (p<0.01) when compared to control group for birds fed diets containing propolis and flavomycin between 14 to 35 days. The addition of 1 g/kg propolis to the diet resulted in significantly (p<0.01) better-feed efficiency as compared to control and other treatment groups. There were no significant differences in carcass yield, abdominal fat, liver gizzard, proventriculus and intestinal weight and intestinal pH among the groups. In addition, serum ALP, total protein, uric acid, cholesterol and triglyceride were not influenced by the any supplementation. However, birds fed with propolis tended to have higher serum HDL and lower level than birds fed the control diet. In conclusion, supplementation of propolis and flavomycin during the growth period showed similar effects on growth performance in quail. Therefore, it can serve as a natiral substitute for antibiotics in poultry diets.


  1. Abdel-Wahhab, M. A., S. A. Nada and F. A. Khalik. 1999. Physiological and toxicological responses in rats fed aflatoxincontaminated diet with or without sorbent materials. Anim. Feed Sci. Techol. 97:209-219.
  2. Alcicek, A., M. Bozkurt and M. Cabuk. 2003. The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. S. Afr. J. Anim. Sci. 33:89-94.
  3. Amaros, M., C. M. O. Simoes, L. Girre, F. Sauvager and M. Cormier. 1994. Comparision of the anti Herpes simplex virus activities of propolis and 3-methyl-butyl -2-enyl caffeate. J. Nat. Prod. 57:644-647.
  4. Anonymous. 1974. Technical publication No. UA4-0160-00, Technion Instr. Corp., Terrytown, 1974. pp. 127-129.
  5. Banskota, A. H., Y. Tezuka, K. Midorikawa, K. Matsushige and S. Kadota. 2000. Two nowel cytotoxic benzofuran derivatives from Brazilian propolis. J. Nat. Prod. 63(9):1277-1279.
  6. Biavatti, M. W., M. H. Bellaver, L. Volpato, C. Costa and C. Bellaver. 2003. Preliminary studies of alternative feed additives for broilers: Alternanthera brasiliana extract, propolis extract and linseed oil. Rev. Bras. Cienc. Avic. 5(2):147-151.
  7. Botsoglou, N. A., E. Christaki, P. Florou-Paneri, I. Giannenas, G. Papageorgiou and A. B. Spais. 2004. The effect of mixture of herbal essential oils or $\alpha$-tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. S. Afr. F. Anim. Sci. 34:52-61.
  8. Demir, E., S. Sarica, M. A. Ozcan and M. Suicmez. 2003. The use of natural feed additives as alternatives for an antibiotic growth promoter in broiler diets. Br. Poult. Sci. 44:44-45.
  9. De-Freitas, R., J. B. Fonseca, R. D. R. N. Soares, H. S. Rostagno and P. R. Soares. 2001. Utilization of garlic (Allium sativum L.) as growth promoter of broilers. Rev. Bras. Zootecnia. 30(3):761-765.
  10. Deschner, E. E., J. Ruperto, G. Wonk and H. L. Newmark. 1991. Quercetin and rutin as inhibitors of azoxymethanol induced colonic neoplasia. Carcinogenesis 12:771-775.
  11. Dimov, V., N. Ivanovska, N. Manolova, V. Bankova, N. Nikolov and S. Popov. 1991. Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie 22:155-162.
  12. Ghisalberti, E. L. 1979. Propolis: a review. Bee world 60:59-84.
  13. Kalavathy, R., N. Abdullah, S. Jalaludin and Y. W. Ho. 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. Br. Poult. Sci. 44(1):139-144.
  14. Keskin, N., S. Hazir, K. H. C. Baser and M. Kurkcuoglu. 2001. Antibacterial activity and chemical composition of Turkish propolis. Z. Naturforsch 56:1112-1115.
  15. Kolonkaya, D., G. Selmanoglu, K. Sorkun and B. Salih. 2002. Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats. Food Chemistry 78:213-217.
  16. Kucukersan, K., S. D. Tuncer, Y. Sanli, M. Midilli, E. Goncuoglu, S. Kucukersan and H. Tan. 2002. The effects of dietary stabilized rumen extract (SRE) and virginamycine on performance and carcass yield of broilers. Revue De Medicine Veterinaire 153(11):723-726.
  17. Kujumgiev, A., I. Tsvetkova, Yu. Serkedjieva, V., Bankova, R. Christov and S. Popov. 1999. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J. Ethnopharmacology 64:235-240.
  18. Manolovan, M., V. Maximova, G. Gegova, Y. Serkedjıeva, S. Uzunov, N. Marekov and V. Bankova. 1985. On the influenza action of fractions from ropolis. Comptes rendus de I’academie de Bulgare de Sciences 38:735-738.
  19. Matsuno, T., Y. Matsumoto, M. Saito and J. Morikawa. 1997. Isolation and characterization of cytotoxic diterpenoid isomers from Propolis. Z. Naturforsch (52c):702-704.
  20. Miyataka, H., M. Nishiki, H. Matsumoto, T. Fujimoto, M. Matsuka and T. Satoh. 1997. Evaluation of propolis. I. Evaluation of Brazilian and Chinese propolis by enzimatic and phsico-chemical methods. Biological and Pharmaceutical Bulletin 20:496-501.
  21. Mohan, B., R. Kadirvel, A. Natarajan and M. Bhaskaran. 1996. Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers. Br. Poult. Sci. 37(2):395-401.
  22. National Research Council. 1994. Nutrient Requirements of Poultry. 9th rev. Ed. National Academy Press.Washington, DC.
  23. Orhan, H., S. Marol, I. F. Hepsen and G. Sahin. 1999. Effects of some probable antiooxidants on selenite-induced cataract formation and oxidative stress-related parameters in rats. Toxicology 139(3):219-232.
  24. Ota, C., C. Unterkircher, V. Fantinato and M. T. Shimuzu. 2001. Antifungal activity of propolis on different species of Candida. Mycoses 44:375-378.
  25. Paintz, M. and J. Metzner. 1979. Zur lokalanasthetischen wirkung vin propolis und einigen inhaltsstofem. Pharmazie 34:839-841.
  26. Pietras, M. 2001. The effect of probiotics on selected blood and meat parameters of broiler chickens. J. Anim. Feed Sci. 10:297-302.
  27. Popova, M., S. Silici, O. Kaftanoglu and V. Bankova. 2005. Antibacterial activity of Turkish propolis and its qualitative and quatitative chemical composition. Phytomedicine (in press).
  28. Revington, B. 2002. Feeding poultry in the post-antibiotic era. Multi-State Poult. Meeting May 14-16. pp. 1-14.
  29. Sawaya, A. C. H. F., A. M. Palma, F. M. Caetano, M. C. Marcucci, I. B. Silvacunha, C. E. P. Araujo and M. T. Shimizu. 2002. Comparative study of in vitro methods used to analyse the activity of propolis extracts with different compositions against species of Candida. Letters in Applied Microbiology 35:203-207.
  30. Sforcin, J. M., J. A. Fernandes, C. Lopes, V. Bankova and S. R. C. Funari. 2000. Seasonal effect on Brazilian propolis antibacterial activity. J. Ethnopharmacology 73:243-249.
  31. Silici, S. and O. Kaftanoglu. 2003. Antimicrobial analysis of propolis samples from different regions of Turkey. Uludag Bee J. 3(3):16-18.
  32. Siriken, B., I. Bayram and A. G. Onol. 2003. Effects of probiotics: alone and in a mixture of Biosacc$^{\circledR}$ plus Zinc Bacitracin on the caecal microflora of Japanase quail. Res. Vet. Sci. 75(1):9-14.
  33. SPSS, Inc. 1999. SPSS for Windows. Release, 10.0, Copright SPSS inc., Chicago, USA.
  34. Sud’ina, G. F., O. K. Mirzoeva and M. A. Pushkareva. 1993. Caffeic acid phenetyl ester as a lypoxigenase inhibitor with antioxidant properties. FEBS Lett. 329:21-24.
  35. Urbanczyk, J., E. Hanczakowska and M. Swiatkiewicz. 2002. Herb mixture as an antibiotic substitute in pig feeding. Medycyna Weterynaryjna 58(11):887-889.
  36. Velikova, M., V. Bankova, K. Sorkun, S. Houcine, I. Tsvetkova and A. Kujumgiev. 2000. Propolis from Mediterranean Region: Chemical composition and antimicrobial activity. Z. Naturforsch 55:790-793.
  37. Verma, A. K., J. A. Johnson, M. N. Gould and M. A. Tanner. 1988. Inhibition of 7,12-dimethylbenz [a] anthracene and N-nitrosomethylurea-induced rat mammary cancer by dietary flavonol quercetin. Cancer Res. 48:5754-5758.

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