Polymorphism of the Promoter Region of Hsp70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in Lymphocytes in Peripheral Blood of Heat Shocked Dairy Cows

  • Cai, Yafei (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Liu, Qinghua (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Xing, Guangdong (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhou, Lei (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Yang, Yuanyuan (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhang, Lijun (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Li, Jing (Center for Molecular Recognition, Columbia University) ;
  • Wang, Genlin (College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2004.07.17
  • Accepted : 2004.11.12
  • Published : 2005.05.01


The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.


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