Anti-inflammatory Effect of Citrus Unshiu

진피(陳皮)의 염증 억제 효과

  • Park, Seong-Heak (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Yun, Sang-Hak (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Young-Mi (Radiology, College of Oriental Medicine, Won-Kwang University) ;
  • Yeom, Seung-Ryong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Young-Dal (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Shin, Byung-Cheul (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
  • 박성혁 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 윤상학 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 권영미 (원광대학교 한의과대학 방사선과) ;
  • 염승룡 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 권영달 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 신병철 (원광대학교 한의과대학 한방재활의학과교실)
  • Received : 2004.12.23
  • Accepted : 2005.01.17
  • Published : 2005.01.30
  • 20 0


Abstract

목적 : 진피(Citus Unshiu, CU)는 실험적으로 항산화, 항알러지, 항종양 효과 등이 있다고 보고되고 있다. 본 연구에서는 비만세포에서 PMA와 A23187에 의하여 유도된 싸이토카인의 증가에 대한 진피 추출물의 억제 효과와 그 기전을 알아보고자 한다. 방법 : PMA+A23187에 의한 싸이토카인 생성에 대한 진피 추출물의 억제효과 기전을 조사하기 위하여 진피를 처리한 후 $IL-1{\beta}$, IL-8, $TNF-{\alpha}$의 생산 및 혈관내피성장인자 (VEGF), 과립구 대식구 군집 자극 인자 (GM-CSF), 저산소 유도 인자(HIF-1)의 발현을 조사하였다. 결과 : 실험에서 PMA와 A23187을 처리한 경우 대조군에 비하여 $IL-1{\beta}$, IL-8, $TNF-{\alpha}$의 생산을 유의하게 증가시켰으나 진피 추출물을 처리한 군에서는 유의하게 억제되었다. 특히 $IL-1{\beta}$의 생성은 $103.7{\pm}5%$, IL-8 생성은 $34.6{\pm}7%$, $TNF-{\alpha}$의 생성은 $85.9{\pm}4.5%$ 억제되었다. (P<0.05). 또한 진피 추출물은 PMA와 A23187에 의하여 증가한 VEGF, GM-CSF, $HIF-1{\alpha}$의 발현 증가를 억제하였다 (약 90.9%의 VEGF, 61.6%의 GM-CSF). 결론 : 이러한 결과는 진피가 염증반응에 대한 HIF-1의 억제자로 작용할 수 있으면, 진피가 비만세포 매개성 염증 질환 치료제의 후보 물질이 될 것으로 사료된다.

Keywords

Citrus unshiu;Anti inflammatories;Vascular endothelial growth factor (VEGF);Granulocyte macrophage colony stimulating factor (GM-CSF);Hypoxia-inducible factor 1

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