Thermophilic Biohydrogen Production from Glucose with a Long-term Operation of CSTR

CSTR의 장기운전을 통한 포도당으로부터의 고온 수소생산

  • Ahn, Yeong-Hee (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Oh, You-Kwan (Department of Chemical and Biochemical Engineering, Pusan National University) ;
  • Park, Sung-Hoon (Department of Chemical and Biochemical Engineering, Pusan National University)
  • 안영희 (한국과학기술원 생명화학공학과) ;
  • 오유관 (부산대학교 화학생명공학과) ;
  • 박성훈 (부산대학교 화학생명공학과)
  • Published : 2005.12.30

Abstract

Thermophilic $H_2$ was produced for 1 year using a bench-scale continuous stirred tank reactor(CSTR). The CSTR was inoculated with anaerobically digested sludge after heat treatment and fed with a glucose-based medium. The reactor showed relatively short start-up period(30 days) and high maximal $H_2$ yield(2.4 mol $H_2/mol$ glucose). Keeping pH 5.0 or less suppressed methanogenic activity. Bacteria affiliated with Thermoanaerobacterium thermosaccharolyticum kept being dominant from approximately 40 days as determined by DGGE. Environmental perturbation(pH or temperature) caused the decrease of biomass concentration in the reactor and the instability of reactor performance, $H_2$ production rate and $H_2$ yield. The unstable performance was accompanied with high concentration of lactate in the effluent. Taken together, the poor recovery of CSTR after perturbations could be partly explained by low biomass concentration and/or metabolic shift of the major population in the CSTR.

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