Inhibition of Cell Migration by Corticotropin-Releasing Hormone (CRH) in Human Natural Killer Cell Line, NK-92MI

Corticotropin-Releasing Hormone (CRH)에 의한 인간 자연 살해 세포(NK-92MI)의 Migration 억제

  • Cheon, So-Young (Department of Life Science, Sookmyung Women's University) ;
  • Bang, Sa-Ik (Department of Plastic Surgery, Samsung Medical Center, Sunhkyunkwan University School of Medicine) ;
  • Cho, Dae-Ho (Department of Life Science, Sookmyung Women's University)
  • 천소영 (숙명여자대학교 이과대학 생명과학과 면역학실험실) ;
  • 방사익 (성균관대학교 의과대학 성형외과학교실) ;
  • 조대호 (숙명여자대학교 이과대학 생명과학과 면역학실험실)
  • Published : 2005.12.30

Abstract

Background: Natural killer (NK) cells are CD3 (-) CD14 (-) CD56 (+) lymphocytes. They play an important role in the body's innate immune response. They can induce spontaneous killing of cancer cells or virus-infected cells via the Fas/Fas ligand or the granzyme/perforin systems. The corticotropin-releasing hormone (CRH) is an important regulator for the body's stress response. It promotes proliferation and migration of various cancer cells through the CRH type 1 receptor under stress, and also inhibits NK or T cell activity. However, the relationship of CRH and NK cell migration to the target has not been confirmed. Herein, we study the effect of CRH on NK cell migration. Methods: We used the human NK cell line, NK-92MI, and tested the expression of CRH receptor type 1 on NK-92MI by RT-PCR. This was to examine the effect of CRH on tumor and NK cell migration, thus NK cells (NK-92MI) were incubated with or without CRH and then each CRH treated cell's migration ability compared to that of the CRH untreated group. Results: We confirmed that CRH receptor type 1 is expressed in NK-92MI. CRH can decrease NK cell migration in a time-/dose-dependent manner. Conclusion: These data suggest CRH can inhibit NK cell migration to target cells.

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