Effect of Hwanggumgung, a Natural Product, on Hair Growth Promotion in C57BL6 Mice

C57BL6 마우스에서 복합생약제인 황금궁의 육모 효과

  • Hue Jin-Joo (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Li Lan (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lyu Sul-Hye (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Baek In-Jeoung (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yon Jung-Min (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Nam Sang-Yoon (College of veterinary Medicine and Research Institute of Veterinary Medicine) ;
  • Yun Young Won (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Hwang Seock-Yeon (Chungbuk National University Hospital) ;
  • Hong Jin Tae (College of Pharmacy, Chungbuk National University) ;
  • Lee Beom Jun (College of veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 허진주 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 리란 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 유설혜 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 백인정 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 연정민 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 남상윤 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 윤영원 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 황석연 (충북대학교병원 진단검사의학과) ;
  • 홍진태 (충북대학교 약학대학) ;
  • 이범준 (충북대학교 수의과대학 및 동물의학연구소)
  • Published : 2005.12.01

Abstract

Hwanggumgung (HGG) is a hair-care product which is composed of several plant extracts used in oriental medicine. This study was carried out to investigate effect of HGG on hair regrowth in a shaving model of C57BL6 mice. Five-week-old mice were acclimated for 1 week under 23$\pm$3$^{\circ}C$, 50$\pm10\%$ relative humidity and 12 h of a light/dark cycle before beginning experiment. There were four experimental groups including distilled water (D.W., control), 10$\%$ ethanol (EtOH, vehicle control), a positive control of 3$\%$ minoxidil (MXD), and HGG for female and male mice, respectively; Six-weeks old mice were trimmed by electric clippers so as not to damage the skin. The next day; mice without visible scraches were selected, randomized and separated in groups of 11 mice. The test compounds were topically treated with 0.15ml per mouse per day for 21 days. The hair regrowth was photographically and histologically determined during the experimental period of 21 days. Enzyme activities of $\gamma$-glutamyl transpeptidase and alkaline phosphatase were also determined using a rate assay method. There were no clinical signs in all experimental groups. The topical application of 3$\%$ MXD and HGG in female mice promoted hair regrowth earlier and faster than the control groups. In male mice, the topical application of 3$\%$ MXD and HGG also accelerated hair growth compared with the controls. Ten percent ethanol also promoted hair growth faster than D.W group. The histology of hair growth in experimental groups was strongly associated with the hair regrowth. 3$\%$ MXD and HGG promoted elongation of hair follicles compared with the controls in both female and male mice. Activities of alkaline phosphatase and $\gamma$-glutamyl transpeptidase, enzymes related to hair growth, significantly increased after treatments of 3$\%$ MXD and HGG for 2 weeks in both female and male mice (p < 0.05). These results suggest that HGG has hair growth promoting activities and it can be for treatment for alopecia.

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