Effect of Chestnut Bark Extracts on Tyrosinase Gene Expression

율피 추출물이 티로시나아제 유전자 발현에 미치는 효과

  • Chin Jong-Eon (Dept. of Cosmetology, Dongkang College) ;
  • Kim Kwan-Chun (Dept. of Enviromental Administration, Gwangju Health College)
  • 진종언 (동강대학 피부미용과) ;
  • 김관천 (광주보건대학 환경행정과)
  • Published : 2005.09.01

Abstract

Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. $10{\mu}g/ml{\ell}\;100{\mu}g/m{\ell}$, $1mg/m{\ell}$ of the extract repressed expression of tyrosinase gene about $38\%,\;47\%,\;and\;78\%$, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at $1{\mu}g/m{\ell},\;10{\mu}g/m{\ell},\;100{\mu}g/m{\ell},\;and\;1mg/m{\ell}$, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at $10{\mu}g/m{\ell}\;and\;100{\mu}g/m{\ell}$.

Keywords

References

  1. Aroca, P., Urabe, K, Kobayashi, K, Taska-moto, K, and Hearing V. J. Melanin biosynthesis patterns of following hormonal stimulation. J. Biol. Chem 268, 25650-25655, 1993
  2. Paval, S. Dynamics of melanogenesis intermediates. J. Invest. Dermatology 100, 162S-165S, 1993 https://doi.org/10.1111/1523-1747.ep12465095
  3. Jimenez-Cervantes, C, Solano, F., Kobayashi, T., Urabe, K, Hearing, V. J., Lozano, J., and Garcia-Borron, C. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269, 17993-18001, 1994
  4. Jung, S. W., Lee, N. K, Kim, S. J., and Han, D. S. Screening of tyrosinase inhibitor from plants. Korean J. Food Sci. Technol. 27, 891-896, 1995
  5. Lee, S. H, Park, J. S, Kim, S. Y, Kim, J. J., and Chung, S.R. The screening of the inhibitory compounds on tyrosinase activity from the natural product. Yakhak Hoeji 41, 456-461, 1997
  6. Jeong, H, Park, Y. G., Shin, U. K, Shin, S. K., Baek, S. K, Lee, M. H., Chung, and Park, Y. I. Tyrosinase Inhibition Activity of some herbal drugs. Yakhak Hoeji 41, 518-523, 1997
  7. Choi, B. W., Lee, B. H, Kang, K. J., Lee, E. S., and Lee, N. H. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Korean J. Pharm. 29, 237-242, 1998
  8. Yang, M. J., Lim, S. J., Ahn, H. S., Kim, M. A., and Ahn, R. M Inhibitory effects of chestnut bark extracts on tyrosinase activity and melanin biosynthesis. Kor. J. Env. Hlth Soc. 25(1), 37-43, 1999
  9. Yang, M. J., Kim, M. G., Lim, S. J., Ahn, H. S., and Ahn, R. M. Inhibitory effects of water-acetone extract of chestnut inner shell, pine needle and hop on the melanin biosynthesis. Yakhak Hoeji 43(4), 494-501, 1999
  10. Baurin, N., Arnoult, E, Scior, T., Do, Q. T., and Bernard, P. Preliminary screening of some tropical plants for anti-tyrosinase activity. J. Ethnopharmacology 82, 155-158, 2002 https://doi.org/10.1016/S0378-8741(02)00174-5
  11. Lee, K. T., Lee, K. S., Jeong, J. H., Jo, B. K., Heo, M. Y., and Kim, H. P. Inhibitory effects of Ramulus mori extracts on melano-genesis, J. Cosmet Sci. 54(2), 133-142, 2003
  12. Koichi Iida, Koji Hase, Kenji Shimomura, Syu Sudo, Shigetoshi Kadota, and Tsuuneo Namba. Potent inhibitors of tyrosinase activity and melanin biosynthesis from Rheum officinale. Planta Medica, 61, 425-428, 1995 https://doi.org/10.1055/s-2006-958129
  13. Isao Kubo, Yoshihiro Yokokawa, and Ikuyo Kinst-Hori. Tyrosinase inhibitors from Bolivian medicinal plants. Journal of Natural products 58, 739-743, 1995 https://doi.org/10.1021/np50119a013
  14. Jang, D. I., Lee, B. G., Jeon, C. O., Jo, N. S., Park, J. H., Cho, S. Y., Lee, H., and Koh, J. S. Melanogenesis inhibitor from paper mulberry. Cosmetics & Toiletries Magazine 112(3), 59-62, 1997
  15. Tomohiro Yokota, Hiroyuki Nishino, Yasuo Kubota, and Masako Mizoguchi. The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation. Pigment Cell Research 11, 355-361, 1998 https://doi.org/10.1111/j.1600-0749.1998.tb00494.x
  16. Lee, S. H., Park, J. S., Kim, S. Y, Kim, J. J., and Kim, S. R. Isolation of inhibitory components on tyrosinase activity from the bark of Paeonia moutan. Yakhak Hoeji 42, 353-358, 1998
  17. Shin, N. H., Ryu, S. Y., Choi, E. J., Kang, S. H., Chang, I. M., Min, K. R., and Kim, Y. S. Oxyresveratrol as the potent inhibitor on the dopa oxidase activity of mushroom tyrosinase. Biochemical and Biophysical Research Communications 243, 801-803, 1998 https://doi.org/10.1006/bbrc.1998.8169
  18. Lee, S. H., Choi, S. Y., Kim, H. C, Hwang, J. S., Lee, B. G, Gao, J. J., and Kim, S. Y. Mulberroside F isolated from the leaves of Moms alba inhibits melanin biosynthesis. Biol. Pharm Bull. 25(8), 1045-1048, 2002
  19. No, J. K., Soung, D. Y., Kim, Y. J., Shim, K. H., Jun, Y. S., Rhee, S. H., Takako Yokozawa and Chung, H. Y. Inhibitory of tyrosinase by green tea components. Life Science 65, PL241-246, 1999 https://doi.org/10.1016/S0024-3205(99)00492-0
  20. Isao Kubo and Ikuyo Kinst-Hori. 2-Hydroxy-4-methoxy-benzaldehyde a potent tyrosinase inhibitor from African medicinal plants. Planta Medica 65, 19-22, 1999 https://doi.org/10.1055/s-1999-13955
  21. Jin, M. H, Kim, H. J., Kang, S. J., Kang, S. H., Kim, C. H., and Jung, M. H. Three melanogenesis Inhibitors from the root of Veratrum nigrum. Kor. J. Pharmacogn 33(4), 395-398, 2002
  22. Kim, C. T, Kim, W. C, Jin, M. H., Kim, H. J., Kang, S. J, Kang, S. H., Jung, M. H., and Lim, Y. H. Inhibitor of melanogenesis from the root of Peucedanum praeruptorum Kor. J. Pharmacogn. 33(4), 395-398, 2002
  23. Kim, J. A., Choi, J. Y., Son, A. R., Park, S. H., Hua, X. G., Lee, J. G., Oh, I. S., Kim, J. J., Chang, H. W., Chung, S. R., Jang, T. S, and Lee, S. H. Inhibitory effects of some natural polyphenols isolated from Euphorbia-ceae plants on melanogenesis. Kor. J. Pharmacogn. 35(2), 157-163, 2004
  24. Kim, C. T., Jung, M. H., Moon, C. S., Lim, Y. H., Kang, S. J., and Cho, W. G. Inhibitor of melanogenesis from Atractylodes rhizo-mas. Kor. J. Pharmacogn 36(1), 60-63, 2005
  25. Son, A. R., Choi, J. Y, Kim, J. A., Cho, S. H., Hua, X. G., Park, S. H, Chung, S. R., Chung, T. C, Jahang, Y. D., Son, J. K., and Lee, S. H. Isolation of melanogenesis inhibitors from Ponciri fructus. Kor. J. Pharmacogn. 36(1), 1-8, 2005
  26. Chin, J. E., Sun, H. S., Lee, K. J., Choi, T. J., Ko, Y. S., Sohn, H. J., Kim, J. J., Jeon, B. H., and Blaise Lee, H. H. Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells. International Journal of Oriental Medicine 1, 6-13, 2000
  27. Cho, N. C, Yoon, Y. H., Lee, H. J., Shon, H. J, Kim, Y. K, Choi, K. H., Ra, M. S., Cho, Y. K, Lee, B. H., and Chin, J. E. Effect of onion(Allium cepa L.) extract on tyrosinase gene expression. Kor. J. Food & Nutr. 14(3), 228-232, 2001
  28. Mosmann, T. J. Rapid colormetric assay for cellular growth and survival Application to proiferation and cytotoxicity assay. Immunol. Methods 63, 55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4