Determination of paraquat-resistant biotype on Conyza canadensis and the resistant mechanism

Paraquat 저항성 생태형 망초의 선발과 저항성 기작

  • Kim, Sung-Eun (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University) ;
  • Kim, Seung-Yong (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University) ;
  • Ahn, Sul-Hwa (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University) ;
  • Chun, Jae-Chul (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University)
  • 김성은 (전북대학교 응용생물공학부 및 생리활성물질연구소) ;
  • 김승룡 (전북대학교 응용생물공학부 및 생리활성물질연구소) ;
  • 안설화 (전북대학교 응용생물공학부 및 생리활성물질연구소) ;
  • 전재철 (전북대학교 응용생물공학부 및 생리활성물질연구소)
  • Published : 2005.03.31

Abstract

Paraquat-resistant biotype of Conyza canadensis (L.) Cronq. was determined by chlorophyll loss and random amplified polymorphic DNA (RAPD) analysis and the resistant mechanism was investigated with respect to absorption, translocation, and binding constant. RAPD analysis for paraquat resistant (R) and susceptible (S) biotypes found in a pear orchard revealed that the biotypes possessed remote genetic relationship. Chlorophyll loss, as an indication of paraquat toxicity, of S biotype was 7.8-fold greater than that of R biotype. There were no differences in contents of epicuticular wax and cuticle and amounts of [14C]paraquat penetrating the cuticle between the two biotypes. Little translocation of the herbicide out of the treated leaf was observed in either biotype. Binding constants of paraquat to the cell wall and thylakoid membrane were 7.4-fold and 16.9-fold, respectively, higher in R biotype than in S biotype. The results suggest that the resistance mechanism of C. canadensis biotype is due partly to high binding affinity of paraquat to the cell wall and thylakoid membrane.

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