Effects of Simulated Acid Rain on Growth and Antioxidant System in French Marigold (Tagetes patula L.)

인공산성비가 만수국(Tagetes patula L.)의 생육 및 항산화 작용에 미치는 영향

  • Kim, Hak-Yoon (Faculty of Environmental Studies, Keimyung University) ;
  • Kim, Jeung-Bea (Faculty of Environmental Studies, Keimyung University)
  • Published : 2005.06.30


This study was conducted to investigate the effect of simulated acid rain (SAR) on growth and antioxidant system in french marigold (Tagetes patula L.). Plants were subjected to four levels of SAR (pH 5.6, 4.0, 3.0, 2.0) in the growth chambers for 2 weeks. SAR decreased both plant height and plant dry weight of french marigold. As the pH levels decreased from 5.6 to 2.0, the content of MDA highly increased linearly. The ratios of dehydroascorbate/ascorbate and oxidized glutathione/reduced glutathione were significantly increased with decreasing pH levels. The enzyme (superoxide dismutase, ascorbate peroxidase etc.) activities of the plant affected by SAR were increased as the pH decreased. Based on the results, SAR caused oxidative stress in french marigold and resulted in significant reduction in plant growth. Biochemical protection responses might be activated to prevent the plant from damaging effects of oxidative stress generated in SAR.


  1. Luxmoore, R. J., Gizzard, T. and Strand, R. H. (1981) Nutrient translocation in the outer canopy and understory of an eastern deciduous forest, For. Sic. 27, 505-518
  2. Haines, B., Stefani, M. and Hendrix, F. (1980) Acid rain: threshold of leaf damage in eight plant species from a southern Appalachian forest succession, Water, Air and Soil, Pollut. 114, 403-407
  3. Nouchi, I. (1991) Acid rain and plant damage, J. Agr. Met. 47, 165-175
  4. Craker, L. E. and Bernstein, D. (1984) Buffering of acid rain by leaf tissue of selected crop plants, Environ. Pollut. 36, 375-381
  5. Binns, W. O. and Redfern, D. B. (1992) Acid rain and forest decline in West Germany, Forestry Conmission Res. Dev. Paper 131, 13
  6. Zedaker, S. M., Nicholas, N. Y. and Eagar, C. (1988) Assesment of forest decline in the Southern Appalanchain surface fir forest, p.334-338. In Bucher, J. B. and Wallim, I. B. (eds.) Air pollution and forest decline, IUFRO, Switzerland
  7. Pylypec, B. and Redmann, R E. (1984) Acid-buffering capacity of foliage from boreal forest species, Can. J. Bot. 62, 2650-2653
  8. Elstner, E. F. (1982) Oxygen activation and oxygen toxicity, Ann. Rev. Plant Physiol. 33, 73-96
  9. Heath, R. L. and Packer, L. (1968) Photoperoxidation in isolated chloroplasts. 1. Kinetic and stoichiometry of fatty acid peroxidation, Arch. Biochem. Biophys. 125, 189-198
  10. Kim, H. Y., Kobayashi, K., Nouchi, I. and Yoneyana, T. (1996) Differential influences of UV-B radiation on antioxidants and related enzymes between rice (Oryza sativa L.) and cucumber (Cucumis sativus L.) leaves, Environ. Sci. 9, 55-63
  11. Gabara, B., Sklodowska, M., Wyrwicka, A., Glinska, S. and Capinska, M. (2003) Changes in the ultrastructure of chloroplasts and mitochondria and antioxidant enzyme activity in Lycopersicon esculentum Mill. leaves sprayed with acid rain, Plant sci. 164, 507-516
  12. Velikova, V., Yordanov, I. and Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants; Protective role of exogenous polyamine, Plant Sci. 151, 59-66
  13. Fan, H. B. and Wang, Y. H. (2000) Effects of simulated acid rain on germination, foliar damage, chlorophyll contents and seedling growth of five hardwood species growing in China, Forest Eco. Manage. 126, 321-329
  14. Singh, A. and Agrawal, M. (1996) Response of . two cultivars of Triticum aestivum L. to simulated acid rain, Environ. Pollut. 91, 161-167
  15. Lichtenthaler, H. K. (1987) Chlorophylls and carotenoids: pigments of photosynthesis, Methods Enzymol. 148, 305-352
  16. Bolin, D. W. and Book, L. (1974) Oxidation of ascorbic acid to dehydroascorbic acid, Science 106, 451
  17. Law, N. Y., Charles, S. A. and Halliwell, B. (1983) Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and paraquat, Biochem. J. 210, 899-903
  18. Tanaka, K., Kondo, N. and Sugahara, K. (1982) Accumulation of hydrogen peroxide in chloroplasts of $SO_2$ fumigated spinach leaves, Plant Cell Physiol. 23, 999-1007
  19. Hossain, M. A., Nakano, Y. and Asada, K. (1984) Monodehydroascorbate reductase in spinach chloro-plasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide, Plant Cell Physiol. 25, 385-395
  20. Tanaka, K. and Sugahara, K. (1980) Role of superoxide dismutase in defense against $SO_2$ oxicity and an increase in superoxide dismutase activity with $SO_2$ fumigation, Plant Cell Physiol. 21, 601-611
  21. Lee, J. J., Neely, G. E., Perrjiean, S. C. and Grothaus, L. C. (1981) Effects of simulated sulfuric acid rain on yield, growth and foliar injury of several crops, Environ. Exp. Bot. 21, 171-185
  22. Huh, H. W. and Huh. M. K. (1998) The effect of simulated acid rain on the growth of important crops, J. Kor. Environ. Sci. 7, 123-131
  23. Schoner, S. and Krause, G. H. (1990) Protective systems against active oxygen species in spinach: response to cold accumulation in excess light, Planta 180, 383-389
  24. Evans, L. S. and Curry, T. M. (1979) Differential response of plant foliage to simulated acid rain, Amer. J. Bot. 66, 953-962