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저선량 감마선 조사가 벼 유묘의 염 스트레스 경감에 미치는 영향

Effects of Low Dose Gamma-irradiation on Alleviation of Salt Stress in Rice (Oryza sativa L.) Seedling

  • 백명화 (한국원자력연구소 방사선이용연구부) ;
  • 김진홍 (한국원자력연구소 방사선이용연구부) ;
  • 위승곤 (한국원자력연구소 방사선이용연구부) ;
  • 이인중 (경북대학교 농학과) ;
  • 이규성 (농촌진흥청 작물과학원) ;
  • 김재성 (한국원자력연구소 방사선이용연구부)
  • Baek, Myung-Hwa (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Hong (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Wi, Seung-Gon (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, In-Jung (Department of Agronomy, Kyungpook National University) ;
  • Lee, Kyu-Seong (National Institute of Crop Science, R.D.A.) ;
  • Kim, Jae-Sung (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 발행 : 2005.06.30

초록

염 스트레스 하에서 벼 생육에 대한 저선량 방사선의 경감효과를 확인하기 위해 일품벼와 간척벼 두 품종에 저선량 감마선을 0-32 Gy 수준으로 조사하였다. 염 처리에 따른 생장반응은 무 처리구에 비해 두 품종 모두 염 처리구에서 생육이 감소하였으며, 초장과 생체중보다 건물중에서 더 많은 감소를 보였다. 이외에도 초기건량에 대한 단위시간당 건물의 증가를 나타내는 상대생장율을 관찰한 결과, 전체 상대생장율이 염 처리구에서 50% 이상 억제되었다. 그러나 이러한 생육억제는 저선량 방사선에 의해 경감됨을 확인할 수 있었다. 특히, 줄기 상대생장율의 경우 염 처리시 4 Gy 조사구가 대조구보다 30% 이상의 경감효과를 보였다. 또한 염 스트레스에 대한 조직의 막투과성 정도는 염 처리에 의해 4배 정도 피해를 보였으나 4 Gy 조사구에서 10% 이상의 경감효과를 보였으며, 상대수분함량도 30% 정도의 감소를 보인 염 처리구에서 저선량 방사선의 효과를 확인 할 수 있었다. 이러한 결과를 통해, 저선량 방사선이 염 스트레스에 대한 저해 효과를 경감시키는 것으로 사료된다.

키워드

Alleviation effects;low dose gamma-irradiation;salt stress;seedling growth;electrolyte leakage;relative water content

참고문헌

  1. Flowers, T. J. and Yeo, A. R. (1995) Breeding for salinity resistance in crop plants: where next?, Aust. J. Plant Physiol. 22, 875-884 https://doi.org/10.1071/PP9950875
  2. Choung, J. I., Yu, S. J., Oh, M. K., Baek, N. H., Ko, J. K. and Lee, J. K. (2002) Varietal responses of rice growth and yield to soil salt content, Kor. J. Crop Sci. 47, 422-426
  3. Lee, I. S. (2002) In vitro selection and characterizations of gamma radiation-induced salt tolerance lines in rice, Ph.D. Thesis, Chungnam National University, Daejon
  4. Balasuburamanian, V. and Rac, S. (1977) Physiology basis of salt tolerance in rice, PI, physiol. section, Tamil Nagada Agri. Univ. India. 26, 291-294
  5. Luckey, T. D. (1980) Hormesis with ionizing radiation, CRC press. Inc. Boca Raton, Florida
  6. Kim, J. S. and Lee, Y. B. (1998) Ionizing radiation hormesis in crops, Kor. J. Environ. Agri. 17, 76-85
  7. Lee, E. K., Kim, J. S., Lee, Y. K. and Lee, Y. B. (1998) Effect of low dose ${\gamma}$-ray irradiation on the germination and growth in red pepper (Capsicum annuum L.), J. Kor. Soc. Hort. Sci. 39, 670-675
  8. Campos, F. F. and Velasco, E. G. (1962) Comparative effect of $cobalt^{60}$ on plant character of two recommended Philippine lowland rice varieties, Philipp. Agric. 46, 93-95
  9. Kaiwdl, K. and Rosner, M. (1965) The accelerating effect of small radiation doses on plant, Bayerisches Landwirtschaftliches Jahrbuch 42, 11-29
  10. Vlasyuk, P. A. (1964) Effect of ionizing radiation on the physiological-biochemical properties and metabolism of agricultural plants, Inst. Fiziol. Biokhim, Rast. SSR. 24-31
  11. Kim, J. S., Lee, E. K., Back, M. H., Park, H. S. and Kim, K. H. (1999) Effect of low dose of gamma radiation on the growth of groundnut (Arachis hypogaea L.), Kor. J. Environ. Biol. 17, 257-261
  12. Kim, J. S., Lee, Y. K., Park, H. S., Back, M. H. and Kim, D. H. (2000) Influence of low dose gamma radiation on the growth of maize (Zea mays L.) varieties, Kor. J. Environ. Agri. 19, 328-331
  13. Kim, J. S., Lee, E. K., Lee, Y. K. and Lee, Y. B. (1999) Influence of artificial acid rain on the growth of soybean irradiated with low dose gamma radiation, Kor. J. Environ. Agri. 18, 245-249
  14. Lee, H. Y., Kim, J. S., Baek, M. H., Park, S. C. and Park, Y. I. (2002) Effects of low dose ${\gamma}$-radiation on photosynthesis of red pepper (Capsicum annuum L.) and the reduction of photoinhibition, Kor. J. Environ. Agri. 21, 83-89 https://doi.org/10.5338/KJEA.2002.21.2.083
  15. Lee, H. Y., Kim, J. S., Baek, M. H., Yoo, J. C. and Kwon, S. T. (2003) Effects of low dose ${\gamma}$ irradiation on physiological activities of radish (Raphanus sativus) during early growth and reduction of ultraviolet-B stress, Kor. J. Horti. Sci. 44, 314-320
  16. Niels, W. H. and Roger, J. B. (1970) Manual on Radiation Dosimetry, Mard Dekker Inc., New York
  17. Barr, H. D. and Weatherley, P. E. (1962) A reexamination of the relative turgidity technique for estimating water deficit in leaves, Aust. J. Biol. Sci. 15, 413-428 https://doi.org/10.1071/BI9620413
  18. Chuan, C. L. and Ching, H. K. (1996) Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl, Plant Sci. 114, 121-128 https://doi.org/10.1016/0168-9452(96)04323-3
  19. Aeschxbacher, R. A., Schiefelbein, J. W. and Benfey, P. N. (1994) The genetic and molecular basis of root development, Ann. Rev. of Plant Physiol. and Plant Mol. BioI. 45, 25-45 https://doi.org/10.1146/annurev.pp.45.060194.000325
  20. Yeo, A. R. and Flowers. T. J. (1982) Accumulation and localization of sodium ions within the shoots of rice (Oryza sativa L.) varieties differing in salinity resistance, Physiol. Plant 56, 189-195 https://doi.org/10.1111/j.1399-3054.1982.tb00323.x
  21. Hwangbo, J. K, Kim, J. S., Lim, J. H., Baek, M. H. and Chung, B. Y. (2003) Alterations in seed vigour and viability of soybean related with accelerated seed aging and low dose gamma irradiation, Kor. J. Crop Sci. 48, 334-338
  22. Kim, J. S., Lee, E. K., Song, J. Y., Kim, H. K. and Lee, Y. B. (2000) Induction of resistance against Phyphthora Blight of pepper by low dose gamma ray radiation, Kor. J. Environ. Biol. 18, 47-51
  23. Dubey, R. S. (1994) Protein synthesis by plants under stressful conditions, In: M. Pessarakli (Ed), Handbook of Plant a Crop Stress, Marcel Dekker, New York. pp 277-299
  24. Shin, S. H. (2002) Physiological and anatomical characteristics of salt-tolerance of rice (Oryza sativa L.) varieties, Ph.D. Thesis, Chonnam National University, Kwangju
  25. Gadallah, M. A. A. (1999) Effect of proline and glycine-betain on Vicia faba responses to salt stress, Biol. Plant. 42, 247-279 https://doi.org/10.1007/BF03030486
  26. Kim, J. S., Lee, Y. K., Back, M. W., Lee, Y. B. and Park, Y. S. (1999) Influence of the low dose ${\gamma}$-ray radiation on the old seed germination and growth of chinese cabbage, Kor. J. Environ. BioI. 17, 11-15
  27. Maribel, L., Dionisio, S. and Satoshi, T. (1998) Antioxidant responses of rice seedlings to salinity stress, Plant Sci. 135, 1-9 https://doi.org/10.1016/S0168-9452(98)00025-9