Effect of Water Extract of Germinated Brown Rice on Adiposity and Obesity Indices in Mice Fed a High Fat Diet

  • Oh, Suk-Heung (Department of Medicinal Biotechnology, Woosuk University, Department of Life Science and Technology, Graduate School, Woosuk University) ;
  • Moon, Yeon-Jeong (Department of Life Science and Technology, Graduate School, Woosuk University) ;
  • Soh, Ju-Ryoun (Department of Food Science and Human Nutrition, and 1nstitute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, and 1nstitute for Molecular Biology and Genetics, Chonbuk National University)
  • Published : 2005.09.01


An extract obtained from germinated brown rice was evaluated for protective effects against the adverse effects of a chronic high fat diet. C57BL6/J mice were divided into three groups; normal diet + water group (ND), high fat diet + water group (HD), high fat diet + brown rice water extract group (HD + BE) and fed for 8 weeks (6 day/week). The ND group diet was 11 kcal fat $\%$. The HD group and HD + BE group diet were 42 kcal fat $\%$. Weight gains were not significantly different between groups. However, abdominal fat $\%$ was 1.6-fold higher in the HD group than HD + BE group. Feed consumption was significantly higher in the ND group than HD group. Serum triglyceride, total cholesterol and LDL-cholesterol increased with a high fat diet, but decreased by brown rice water extract administration to the high fat diet group. Serum HDL-cholesterol decreased by the high fat diet, but increased by the brown rice wate extract administration. Also, serum HDL-cholesterol/total cholesterol ratio was 2.3-fold higher in the HD + BE than the HD. Liver triglyceride and total cholesterol were not significantly different among groups. These data suggest that brown rice water extract administration improves the serum lipid profiles of C57BL/6J mice.


  1. Huh KB. 1990. The present status of nutrition-related diseases and its countermeasures. Kor J Nutr 23: 197-207
  2. Lee HG. 1996. Pattern of disease incidence and nutrition in Korea. Kor J Nutr 29: 381-383
  3. Oh SH. 2003. Stimulation of $\gamma$ -aminobutyric acid synthesis activity in brown rice by a chitosan/glutamic acid germination solution and calcium/calmodulin. J Biochem Mol Biol 36: 319-325
  4. Laboure AM, Gangnon J, Lescure AM. 1993. Purification and characterization of a phytase (myo-inositol-hexakisphosphate phospho-hydrolase) accumulated in maize (Zea mays) seedlings during germination. Biochem J 295: 413-419
  5. Satyanarayan V, Nair PM. 1990. Metabolism enzymology and possible roles of 4-aminobutyrate in higher plants. Phytochem 29: 367-375
  6. Omori M, Yano T, Okamoto T, Tsushida T, Murai T, Higuchi M. 1987. Effect of anaerobically treated tea (Gabaron tea) on blood pressure of spontaneously hypertensive rats. Nippon Nogikagaku Kaishi 61: 1449-1451
  7. Nakagawa K, Onota A. 1996. Accumulation of $\gamma$ -aminobutyric acid (GABA) in the rice germ. Shokuhin Kaihatsu 31: 43-46
  8. Cha YS, Oh SH. 2000. Investigation of $\gamma$ -aminobutyric acid in Chinese cabbages and effects of the cabbage diets on lipid metabolism and liver function of rats administered with ethanol. J Korean Soc Food Sci Nutr 29: 500-505
  9. Oh SH, Cha YS. 2001. Effect of diets supplemented with pharbitis seed powder on serum and hepatic lipid levels, and enzyme activities of rats administered with ethanol chronically. J Biochem Mol Biol 34: 166-171
  10. Oh SH, Soh JR, Cha YS. 2003. Germinated brown rice extract shows a nutraceutical effect in the recovery of chronic alcohol-related symptoms. J Med Food 6: 115-121
  11. Oh SH, Oh CH. 2003. Brown rice extracts with enhanced levels of GABA stimulate immune cells. Food Sci Biotechnol 12: 248-252
  12. Baum G, Lev-Yadun S, Fridmann Y, Arazi T, Katsnelson H, Zik M, Fromm H. 1996. Calmodulin binding to glutamate decarboxylase is required for regulation of glutamate and GABA metabolism and normal development in plants. EMBO J 15: 2988-2996
  13. Cohen SA, Michaud DP. 1993. Synthesis of a fluorescent derivatizing reagent, 6-aminoquinonyl-N-hydroxysuccinimidyl carbonate, and its application for the analysis of hydrolysate amino acids via high performance liquid chromatography. Anal Biochem 211: 279-287
  14. Warnick JB, Benderson J, Albers JJ. 1978. HDL precipitation by dextran sulfate-$MgCl_2$ method. Clin Chem 28: 1379-1383
  15. Friedwald WT, Levy RL, Fredrickso DS. 1972. Estimation of the concentration of low density lipoprotein cholesterol without use of the preparation ultracentrifuge. Clin Chem 18: 499-502
  16. Frings CS, Dunn RT. 1970. A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am J Clin Path 53: 89-91
  17. Folch J, Lees M, Sloane-Stanley GHS. 1983. Simple method for the isolation of total lipids from animal tissues. J Biol Chem 22: 497-509
  18. Duncan DB. 1993. Multiple range test for correlated and heteroscedastic means. Biometrics 13: 164-176
  19. Aubert R, Saguet JP, Lemonnier DL. 1982. Term morphological and metabolic effects of early under- and overnutrition in mice. J Nutr 110: 649-661
  20. Armand M, Borel P, Cara L, Serft M, chautan M, Lafont H, Lairon D. 1990. Adaption of lingual lipase to dietary fat in rats. J Nutr 120: 1148-1156
  21. Faust IM, Johnson PR, Stern JS, Hirsch J. 1978. Dietinduced adipocyte number increase in adult rats: a new model of obesity. Am J Physiol 235: 279-286
  22. Choi JS, Lee JH, Young HS. 1995. Anti-hyperlipidemic effect of phragmites communis and its active priniciples. J Korean Soc Food Nutr 24: 523-529
  23. Ha TY, Kim NY. 2003. The effects of uncooked grains and vegetables with mainly brown rice on weight control and serum components in Korean overweight/obese female. Kor Nutr Soc 36: 183-190
  24. Kim MK, Won EJ. 1984. Effects of feeding polished or brown rice diet with different kinds of lipids on the metabolism in rats. Kor J Nutr 17: 154-162
  25. Goldstein JL, Brown MS. 1983. Lipoprotein receptors: Genetic defense against atherosclerosis. Clin Res 30: 417-423
  26. Steinberg D, Witztum JL. 1990. Lipoproteins and atherogenesis. JAMA 264: 3047-3052
  27. Venter CS, Vorster HH, Van der Nest DG. 1990. Comparison between physiological effects of konjac glucomannan and propionate in baboons fed 'Western' diets. J Nutr 120: 1046-1050
  28. Oh SH, Choi WG. 2000. Production of the quality germinated brown rice containing high $\gamma$ -aminobutyric acid by chitosan application. Korean J Biotechnol Bioeng 15: 615-620
  29. Oh SH, Kim SH, Moon YJ, Choi WG. 2002. Changes in the levels of $\gamma$ -aminobutyric acid and some amino acids by application of a glutamic acid solution for the germination of brown rice. Korean J Biotechnol Bioeng 17: 49-53
  30. Soh JR, Cha YS. 2004. Gamma-aminobutyric acid and/or carnitine supplementation alters lipid and some immune related nutrient levels in mice. J Food Sci Nutr 9: 58-64
  31. Bartnick M, Szafranska J. 1987. Change in phytate content and phytase during the germination of some cereals. J Cereal Sci 5: 23-28
  32. Oh CH, Oh SH. 2004. Effect of geminated brown rice extract with enhanced levels of GABA on cancer cell proliferation and apoptosis. J Medi Food 7: 19-23

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