DOI QR코드

DOI QR Code

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity of Elk (Cervus elaphus) Velvet Antler

  • Karawita Rohan (Faculty of Applied Marine Science, Cheju National University) ;
  • Park, Pyo-Jam (Department of Biotechnology, Konkuk University) ;
  • Siriwardhana Nalin (Faculty of Applied Marine Science, Cheju National University) ;
  • Jeon, Byong-Tae (Nokyong Research Center, Konkuk University) ;
  • Moon, Sang-Ho (Nokyong Research Center, Konkuk University) ;
  • Ahn, Duk-Kyun (Jaseng Research Institute of Bio-Technology & Bioscience) ;
  • Chos, Somi-K. (Faculty of Biotechnology, Cheju National University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
  • Published : 2005.09.01

Abstract

Angiotensin I-converting enzyme (ACE) inhibitory activities of elk antler hydrolysates prepared with three kinds of proteases, pepsin, trypsin and $\alpha-chymotrypsin$, were investigated. The ACE inhibitory activity of the pepsinolytic hydrolysate was the highest with an $IC_{50}$ value of $9.3\mu g/mL.$ In addition, three kinds of pepsinolytic hydrolysates with relatively high molecular weights (over 10,000 Da), medium molecular weights (5,000 to 10,000 Da), and low molecular weights (below 5,000 Da) were fractionated using an ultrafiltration membrane system. The below 5,000 Da hydrolysate exhibited the highest ACE inhibitory activity. These results indicate that the pepsinolytic hydrolysates of elk velvet antler could be a good source of peptides with ACE inhibitory activity.

References

  1. Ondctti MA, Rubin B, Cushman DW. 1977. Design of specific inhibitors of angiotensin converting enzyme: a new class of orally active antihypertensive agents. Sci 196: 441-444 https://doi.org/10.1126/science.191908
  2. Yang HYT, Erdos EG, Levin Y. 1970. A dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin. Biochim Biophys Acta 214: 374-376 https://doi.org/10.1016/0005-2795(70)90017-6
  3. Raia JJ Jr, Barone JA, Byerly WG, Lacy CR. 1990. Angiotensin-converting enzyme inhibitors: a comparative review. The Annuals of Pharmacotherapy 24 5: 506-525
  4. Brown NJ, Vaughan DE. 1998. Angiotensin-converting enzyme inhibitors. Circulation 9714. p 1411-1420
  5. Antonios TF, MacGregor GA. 1995. Angiotensin converting enzyme inhibitors in hypertension: potential problems. J Hvpertens (Suppl) 13: 11-16
  6. Abubakar A, Saito T, Kitazawa H, Kawai Y, ltoh T. 1998. Structural analysis of new antihypertensive peptides derived from cheese whey protein by proteinase K digestion. J Dairy Sci 81: 3131-3138 https://doi.org/10.3168/jds.S0022-0302(98)75878-3
  7. Maeno M, Yamamoto N, Takano T. 1996. Identification of an antihypertensive peptide from casein hydrolysate produced by a proteinase from Lactobacillus helveticus CP790. J Dairy Sci 79: 1316-1321 https://doi.org/10.3168/jds.S0022-0302(96)76487-1
  8. Miyoshi S, Ishikawa H, Kaneko T, Fukui F, Tanaka H, Maruyama S. 1991. Structure and activity of angiotensinconverting enzyme inhibitors in an $\alpha$ -zein hydrolysate. Agric Biol Chem 55: 1313-1318 https://doi.org/10.1271/bbb1961.55.1313
  9. Kohama Y, Matsumoto S, Oka H, Teramoto T, Okabe M, Mimura T. 1988. Isolation of angiotensin-converting enzyme inhibitory from tuna muscle. Biochem Biophys Res Commun 155: 332-337 https://doi.org/10.1016/S0006-291X(88)81089-1
  10. Ukeda H, Matsuda H, Osajima K, Matsufuji H, Matsui T, Osajima Y. 1992. Peptides from peptic hydrolyzate of heated sardine meat that inhibit angiotensin I-converting enzyme. Nippon Nogeikagaku Kaishii 66: 25-29 https://doi.org/10.1271/nogeikagaku1924.66.25
  11. Suh HJ, Whang JH. 1999. A peptide from com gluten hydrolysate that is inhibitory toward angiotensin I-converting enzyme. Biotechnol Let 21: 1055-1058 https://doi.org/10.1023/A:1005688627350
  12. Hyun CK, Shin HK. 2000. Utilization of bovine blood plasma proteins for the production of angiotensin I-converting enzyme inhibitory peptides. Proc Biochem 36: 65-71 https://doi.org/10.1016/S0032-9592(00)00176-X
  13. Kinoshita E, Yamakoshi J, Ikuchi M. 1993. Purification and indentification of an antiotensin I-converting enzyme inhibitor from soy sauce. Biosc Biotechnol Biochem 57: 1107-1110 https://doi.org/10.1271/bbb.57.1107
  14. Ha YW, Jeon BT, Moon SH, Kim YS. 2003. Comparison of biochemical components among different fodders-treated antlers. Kor J Harmacogn 34: 40-44
  15. Wong S. 1991. Velvet antlers for medicine. In Wildlife production: conservation and sustainable development. Renecker LA, Hudson RJ, eds. AFES Misc. Pub. 91-6, University of Alaska, Fairbanks, Alaska. p 530-532
  16. Cushman DW, Cheung HS. 1971. Spectrophotometric assay and properties of the angiotensinconverting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  17. Watanabe T, Mazumder TK, Nagai S, Tsuji K, Terabe S. 2003. Analysis method of the Angiotensin-1 converting enzyme inhibitory activity based on micellarelecrokinetic chromatography. Anal Sci 19: 159-161 https://doi.org/10.2116/analsci.19.159
  18. Lowry OH, Rosebrough JN, Farr AL, Randall RJ. 1951. Protein measurements with the Folin reagent. J Biol Chem 193: 265-275
  19. Sunwoo HH, Nakano T, Hudson RJ, Sim JS. 1995. Chemical composition of antlers from wapiti (Cervus elaphus). J Agric Food Chem 43: 2846-2849 https://doi.org/10.1021/jf00059a014
  20. Cheung HS, Wang FL, Ondetti A, Sabo EF, Cushman DW. 1980. Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. J Biol Chem 255: 401-407
  21. Byun HG, Kim SK. 2001. Purification and cgaracterization of angiotensin I-converting enzyme (ACE) inhibitory activity from Alaska Pollack (Theranga chalcogramma) skin. Proc Biochem 36: 1155-1162 https://doi.org/10.1016/S0032-9592(00)00297-1
  22. Jang A, Lee M. 2004. Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates. Meat Sci 69: 653-661 https://doi.org/10.1016/j.meatsci.2004.10.014
  23. Hazato T, Kase R. 1986. Isolation of angiotensin-converting enzyme inhibitor from porcine plasma. Biochem Biophys Res Commun 139: 52-55 https://doi.org/10.1016/S0006-291X(86)80078-X
  24. Ahn DK, Kim HC, Lee BN. 1999. Effect of deer antler on the blood pressure and heart rate of SHR and S.D. rats. Kor J Herbology 14: 149-152
  25. Matsui T. 2003. Production of hypotensive peptide, SVY, from 7S globulin of soybean protein and its physiological functions. Soy Protein Res 6: 73-77
  26. Matsui T, Li CH, Tanaka T, Maki T, Osajima Y, Matsumoto K. 2000. Depressor effect of wheat germ hydrolysate and its novel angiotensin I-converting enzyme inhibitory peptide, Ile- Val- Tyr, and the metabolism in rat and human plasma. Biol Pharm Bull 23: 427-431 https://doi.org/10.1248/bpb.23.427
  27. Masuda O, Nakamura Y, Takano T. 1996. Antihypertensive peptides are present in aorta after oral administration of sour milk containing these peptides to spontaneously hypertensive rats. J Nutr 126: 3063-3068
  28. Fujita H, Yoshikawa M. 1999. LKPNM: a prodrug-type ACE-inhibitory peptide derived from fish protein. lmmunopharmacol 126: 3063-3068
  29. Lee BY, Lee OH, Chi HS. 2003. Analysis of food components of Korean deer antler parts. Kor J Food Sci Technol 35: 52-56
  30. Arihara K, Nakashima Y, Mukai T, Iahikawa T, Itoh M. 2001. Peptide inhibitors for angiotensin I-converting enzyme from enzymatic hydrolysates of porcine skeletal muscle proteins. Meat Sci 57: 319-324 https://doi.org/10.1016/S0309-1740(00)00108-X
  31. Vermeirssen V, Deplancke B, Tappenden KA, Van Camp J, Gaskins HR, Verstraete W. 2002. Intestinal transport of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg through a Caco-2 Bbe monolayer. J Pept Sci 8: 95-100
  32. Kannel WB. 1996. Blood pressure as a cardiovascular risk factor. J Am Med Assoc 275: 1571-1576 https://doi.org/10.1001/jama.275.20.1571

Cited by

  1. Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by antler and fermented antler using Cordyceps militaris vol.133, pp.2, 2011, https://doi.org/10.1016/j.jep.2010.10.047
  2. Effect of phlorotannins isolated fromEcklonia cavaon angiotensin I-converting enzyme (ACE) inhibitory activity vol.5, pp.2, 2011, https://doi.org/10.4162/nrp.2011.5.2.93
  3. ) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model vol.2018, pp.2090-9071, 2018, https://doi.org/10.1155/2018/6705156