DOI QR코드

DOI QR Code

Analysis of the Activated Sludge of a Municipal WWTP by Several Bio-Parameters

  • Cho Sun-Ja (Department of Microbiology, Pusan National University) ;
  • Jung Yong-Ju (Department of Microbiology, Pusan National University) ;
  • Park Tae-Joo (Department of Environmental Engineering, Pusan National University) ;
  • Lee Sang-Joon (Department of Microbiology, Pusan National University)
  • Published : 2005.09.01

Abstract

The activated sludge from the aeration basin of the Su-yeong municipal wastewater treatment plant which has operated by a standard activated sludge process in Busan, Korea was investigated during April 2004 and January 2005 with several bio-indicators. The number of bacteria and fungi per gram of dry weight of MLSS were estimated to be $3.1\times10^6\sim1.5\times10^8\;and\;l.1\times10^3\sim1.1\times10^5$ colony forming units, respectively, by the plate agar method. By cultivation-independent methods, such as 4',6-diamidino-2-phenylindole stain and fluorescence in situ hybridization, the ratio of eubacteria to the entire biomass was evaluated by more than $80\%$ (v/v). The ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to the total eubacteria was detennined to be $7.0\sim9.8\%\;and\;3.3\sim6.2\%$ without heavy variation in spite of a period of relatively low temperature in the basin. It would be expected that the nitrification would occur or at least co-exist throughout the year in the sludge of many municipal WWTP with influents that contain the sufficient nitrogen sources although the WWTP does not have any specialized processes for the removal of nitrogen.

Keywords

Activated sludge;Wastewater treatment plant (WWTP);Cultivation independent method

References

  1. Dignae, M. F., P. Glnestet, D. Rybacki, A. Bruchet, V. Urbain and P. Scribe, 2000, Fate of wastewater organic pollution duringactivated sludge treatment: nature of residual organic matter, Wat. Res., 34, 4185-4194 https://doi.org/10.1016/S0043-1354(00)00195-0
  2. Bond, P. L., P. Hugenoltz, J. Keller and L. L. Blackall, 1995, Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors, Appl. Environ. Microbiol., 61, 1910-1916
  3. Mitsuyo, H., M. Kamamoto, M. Yani and M. Shoda, 2001, Comparison of the biological NH3 removal characteristics among four inorganic packing materials, J. Biosci. Biotechnol., 91, 428-430
  4. Ulrike, P., A. Pommerening-Roser, S. Juretschko, M. C. Schmid, H. P. Koops and M. Wagner, 2000, Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys, Appl. Environ. Microbiol., 66, 5368-5382 https://doi.org/10.1128/AEM.66.12.5368-5382.2000
  5. Yoshiteru, A. T. M., T. Okamoto, S. Tsuneda, A. Hirata, A. Kitayama and T. Nagamune, 2000, Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hyridization, J. Biosci. Bioeng., 90, 234-240 https://doi.org/10.1016/S1389-1723(00)80075-4
  6. Laborotaries, D., 1984, Difco Manual 10th (ed), Difco, U.S.A., 690pp
  7. Egli, K., A. J. B. Zehnder, M. Wagner and J. R. Meer, 2003, Community analysis of ammonia and nitrite oxidizer during start-up of nitrification reactors, Appl. Environ. Microbiol., 69, 3213-3222 https://doi.org/10.1128/AEM.69.6.3213-3222.2003
  8. APHA, AWWA, WPCF, 1992, Standards methods for the examination of water and wastewater, 8th ed. American Public Health Association, Washington D.C., U.S.A
  9. Amann, R. and W. Ludwig, 2000, Ribosomal RNAtargeted nucleic acid probes for studies in microbial ecology, FEMS Microbiol. Rev., 24, 555-565 https://doi.org/10.1111/j.1574-6976.2000.tb00557.x
  10. Daims, H., U. Purkhold, L. Bjerrum, E. Arnold, P. A. Wilderer, and M. Wagner, 2001, Nitrification in sequencing biofilm batch reactors: lessons from molecular approachs, Wat. Sci. Technol., 43, 9-18
  11. Kampfer, P., P. Kampfer, R. Erhart, C. Beimfohr, J. Bohringer, M. Wagner and R. Amann, 1996, Characterization of bacterial communities from activated sludge, culture-dependent numerical identification versus in situ identification using groupand genus-specific rRNA-targeted oligonucleotide probes, Microbioal Ecol., 32, 101-121
  12. Morris, C. E., M. Bardin, O. Berge, P. Frey-Klett, N. Fromin, H. Girardin, M. H. Guinebretiere, P. Lebaron, J. M. Thiery and M. Troussellier, 2002, Microbial biodiversity: approaches to experimental design and hypothesis testing in primary scientific literature from 1975 to 1999, Microbiol. Mol. Biol. Rev., 66, 592-616 https://doi.org/10.1128/MMBR.66.4.592-616.2002
  13. Ahn, Y. H. and S. H. Park, 2003, Microbial and physicochemical monitoring of granular sludge during start-up of thermophilic UASB reactor, J. Microbiol. Biotechnol., 13, 378-384
  14. Mobarry, B. K., M. Wagner, V. Urbain, B. E. Rittmann and D. A. Stahl, 1996, Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria, Appl. Environ. Microbiol., 62, 2156-2162
  15. Hicks, R., R. Amann and D. Stahl, 1992, Dual staining of natural bacterioplankton with 4',6- diamidino-2-phenylindol and fluorescent oligonuleotide probes targeting kingdom-level 16S rRNA sequences, Appl. Environ. Microbiol., 58, 2158-2163
  16. Berthouex, P. M. and L. C. Brown, 1994, Statistics for environmental engineers, Lewis Publishers, U.S.A., 273-284pp
  17. Fernando, M. S. and D. G. Allen, 2003, Effects of temperature conditions on aerobic biological treatment of wastewater, Wat. Res., 37, 3590-3601 https://doi.org/10.1016/S0043-1354(03)00270-7
  18. Lee, H. W., S. Y. Lee, J. W. Lee, J. B. Park, E. S. Choi and Y. K. Park, 2002, Molecular characterization of microbial community in nitrate-removing activated sludge, FEMS Microbiol. Ecol., 41, 85-94 https://doi.org/10.1111/j.1574-6941.2002.tb00969.x
  19. Cervantes, F. J., D. A. Rosa and J. Gernez, 2001, Nitrogen removal from wastewater at low C/N ratios with ammonium and acetate as electron donors, Bioresource Technol., 79, 165-170 https://doi.org/10.1016/S0960-8524(01)00046-3